SAICAR synthetase: Difference between revisions
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<StructureSection load=' | <StructureSection load='' size='350' side='right' caption='SAICAR synthetase complex with aminoimidazole-ribonucleotide, ADP, TRIS, aspartate, acetate, Mg+2 and Cl- ions (PDB code [[4fe2]]) ' scene='74/749400/Cv/1'> | ||
== Function == | == Function == | ||
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== Structural highlights == | == Structural highlights == | ||
SAI structure shows two domains. | SAI structure shows <scene name='74/749400/Cv/10'>two domains</scene>. The <scene name='74/749400/Cv/11'>active site is located in a hydrophilic tunnel</scene> between the 2 domains and contains <scene name='74/749400/Cv/12'>ADP</scene>, <scene name='74/749400/Cv/13'>aspartic acid</scene> and <scene name='74/749400/Cv/14'>aminoimidazole-ribonucleotide (AIR)</scene><ref>PMID:24598753</ref>. Residues are colored according to {{Template:ColorKey_Hydrophobic}}, {{Template:ColorKey_Polar}}. Water molecules are shown as red spheres. | ||
*<scene name='74/749400/Cv/15'>Surface representation of hydrophilic tunnel</scene>. | |||
*<scene name='74/749400/Cv/16'>Mg coordination site</scene>. | |||
*<scene name='74/749400/Cv/17'>Whole active site</scene>. | |||
</StructureSection> | </StructureSection> | ||
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**[[1a48]] – ySAI - yeast<br /> | **[[1a48]] – ySAI - yeast<br /> | ||
**[[3r9r]] – | **[[3r9r]], [[6yvq]] – MaSAI – ''Mycobacterium abscessus'' <br /> | ||
**[[3kre]] – SAI – ''Ehrlichia chaffeensis'' <br /> | **[[3kre]] – SAI – ''Ehrlichia chaffeensis'' <br /> | ||
**[[1kut]] – SAI – ''Thermotoga maritima''<br /> | **[[1kut]] – SAI – ''Thermotoga maritima''<br /> | ||
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**[[2gqr]] – EcSAI + ADP – ''Escherichia coli''<br /> | **[[2gqr]] – EcSAI + ADP – ''Escherichia coli''<br /> | ||
**[[2gqs]] – EcSAI + ADP + AICAR <br /> | **[[2gqs]] – EcSAI + ADP + AICAR <br /> | ||
**[[6yy6]], [[6yy7]], [[6yy8]], [[6yy9]], [[6yya]], [[6yyb]], [[6yyc]], [[6yyd]], [[6z0q]], [[6z0r]] – MaSAI + inhibitor<br /> | |||
**[[6yx3]] – MaSAI + ATP <br /> | |||
*SAICAR synthetase type-1 | *SAICAR synthetase type-1 | ||
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== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | |||
Latest revision as of 14:18, 9 March 2022
FunctionSAICAR synthetase (SAI) or phosphoribosylaminoimidazole-succinocarboxamide synthetase is part of the purine biosynthesis. SAI catalyzes ATP-dependent ligation of carboxyaminoimidazole ribotide (AICAR) with aspartate[1]. Structural highlightsSAI structure shows . The between the 2 domains and contains , and [2]. Residues are colored according to Hydrophobic, Polar. Water molecules are shown as red spheres.
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3D structures of SAICAR synthetase3D structures of SAICAR synthetase
Updated on 09-March-2022
ReferencesReferences
- ↑ Manjunath K, Jeyakanthan J, Sekar K. Catalytic pathway, substrate binding and stability in SAICAR synthetase: A structure and molecular dynamics study. J Struct Biol. 2015 Jul;191(1):22-31. doi: 10.1016/j.jsb.2015.06.006. Epub 2015, Jun 10. PMID:26072057 doi:http://dx.doi.org/10.1016/j.jsb.2015.06.006
- ↑ Wolf NM, Abad-Zapatero C, Johnson ME, Fung LW. Structures of SAICAR synthetase (PurC) from Streptococcus pneumoniae with ADP, Mg(2+), AIR and Asp. Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):841-50. doi:, 10.1107/S139900471303366X. Epub 2014 Feb 22. PMID:24598753 doi:http://dx.doi.org/10.1107/S139900471303366X