Rop protein: Difference between revisions
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<StructureSection load='1rop' size='350' side='right' scene='' caption='E. coli Rop protein [[1rop]]'> | |||
<scene name='Rop_protein/Wt_rop/1'>Rop</scene> '''(Repressor Of Primer)''' is a small homodimeric RNA-binding protein that is involved in the regulation of copy number of the ColE1 plasmids of E.coli, where it is encoded<ref>pmid 2462471</ref>. Its structure has been studied using both X-ray crystallography | ==Function== | ||
<scene name='Rop_protein/Wt_rop/1'>Rop</scene> '''(Repressor Of Primer)''' is a small homodimeric RNA-binding protein that is involved in the regulation of copy number of the ColE1 plasmids of E.coli, where it is encoded<ref>pmid 2462471</ref>. Its structure has been studied using both X-ray crystallography<ref>PMID:3681971</ref> and NMR<ref>PMID:1841691</ref>. | |||
Each monomer has molecular weight of about 7.500 Da and it consists of 63 amino acids that form two α-helices connected by <scene name='Rop_protein/Wt_rop_loop/2'> a loop </scene>of four amino acids (L29, D30, A31, D32). The two monomers are related with a 2-fold symmetry axis. | Each monomer has molecular weight of about 7.500 Da and it consists of 63 amino acids that form two α-helices connected by <scene name='Rop_protein/Wt_rop_loop/2'> a loop </scene>of four amino acids (L29, D30, A31, D32). The two monomers are related with a 2-fold symmetry axis. | ||
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*re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle([[1yo7]]) | *re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle([[1yo7]]) | ||
*ALA2ILE2-6, repacted the hydrophobic core and a new fold ([[1f4n]]) | *ALA2ILE2-6, repacted the hydrophobic core and a new fold ([[1f4n]]) | ||
</StructureSection> | |||
==3D structures of Rop protein== | ==3D structures of Rop protein== | ||
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[[1rpo]], [[1rop]], [[2ghy]] – EcRop – ''Escherichia coli''<br /> | [[1rpo]], [[1rop]], [[2ghy]] – EcRop – ''Escherichia coli''<br /> | ||
[[1qx8]], [[1nkd]], [[1b6q]], [[1yo7]], [[1f4n]], [[3k79]], [[2ijh]], [[2iji]], [[2ijj]], [[2ijk]], [[1gmg]], [[1f4m]], [[4do2]], [[1gto]] – EcRop (mutant)<br /> | [[1qx8]], [[1nkd]], [[1b6q]], [[1yo7]], [[1f4n]], [[3k79]], [[2ijh]], [[2iji]], [[2ijj]], [[2ijk]], [[1gmg]], [[1f4m]], [[4do2]], [[1gto]], [[7kae]] – EcRop (mutant)<br /> | ||
[[1rpr]] – EcRop – NMR<br /> | [[1rpr]] – EcRop – NMR<br /> | ||
[[3q5z]] – TgRop5B pseudokinase domain – ''Toxoplasma gondii'' <br /> | [[3q5z]] – TgRop5B pseudokinase domain – ''Toxoplasma gondii'' <br /> | ||