7rtt: Difference between revisions
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==Cryo-EM structure of a TTYH2 cis-dimer== | ==Cryo-EM structure of a TTYH2 cis-dimer== | ||
<StructureSection load='7rtt' size='340' side='right'caption='[[7rtt]]' scene=''> | <StructureSection load='7rtt' size='340' side='right'caption='[[7rtt]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTT FirstGlance]. <br> | <table><tr><td colspan='2'>[[7rtt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTT FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtt OCA], [https://pdbe.org/7rtt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtt RCSB], [https://www.ebi.ac.uk/pdbsum/7rtt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtt ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ttyh2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rtt OCA], [https://pdbe.org/7rtt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rtt RCSB], [https://www.ebi.ac.uk/pdbsum/7rtt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rtt ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[[https://www.uniprot.org/uniprot/TTYH2_MOUSE TTYH2_MOUSE]] Probable large-conductance Ca(2+)-activated chloride channel. May play a role in Ca(2+) signal transduction. May be involved in cell proliferation and cell aggregation (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tweety homologs (TTYHs) comprise a conserved family of transmembrane proteins found in eukaryotes with three members (TTYH1-3) in vertebrates. They are widely expressed in mammals including at high levels in the nervous system and have been implicated in cancers and other diseases including epilepsy, chronic pain, and viral infections. TTYHs have been reported to form Ca(2+)- and cell volume-regulated anion channels structurally distinct from any characterized protein family with potential roles in cell adhesion, migration, and developmental signaling. To provide insight into TTYH family structure and function, we determined cryo-EM structures of Mus musculus TTYH2 and TTYH3 in lipid nanodiscs. TTYH2 and TTYH3 adopt a previously unobserved fold which includes an extended extracellular domain with a partially solvent exposed pocket that may be an interaction site for hydrophobic molecules. In the presence of Ca(2+), TTYH2 and TTYH3 form homomeric cis-dimers bridged by extracellularly coordinated Ca(2+). Strikingly, in the absence of Ca(2+), TTYH2 forms trans-dimers that span opposing membranes across a ~130 A intermembrane space as well as a monomeric state. All TTYH structures lack ion conducting pathways and we do not observe TTYH2-dependent channel activity in cells. We conclude TTYHs are not pore forming subunits of anion channels and their function may involve Ca(2+)-dependent changes in quaternary structure, interactions with hydrophobic molecules near the extracellular membrane surface, and/or association with additional protein partners. | |||
Structures of tweety homolog proteins TTYH2 and TTYH3 reveal a Ca(2+)-dependent switch from intra- to intermembrane dimerization.,Li B, Hoel CM, Brohawn SG Nat Commun. 2021 Nov 25;12(1):6913. doi: 10.1038/s41467-021-27283-8. PMID:34824283<ref>PMID:34824283</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7rtt" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Brohawn | [[Category: Lk3 transgenic mice]] | ||
[[Category: Hoel | [[Category: Brohawn, S G]] | ||
[[Category: Li B]] | [[Category: Hoel, C M]] | ||
[[Category: Li, B]] | |||
[[Category: Cisdimer]] | |||
[[Category: Membrane protein]] | |||
[[Category: Ttyh2]] | |||
Latest revision as of 10:26, 9 March 2022
Cryo-EM structure of a TTYH2 cis-dimerCryo-EM structure of a TTYH2 cis-dimer
Structural highlights
Function[TTYH2_MOUSE] Probable large-conductance Ca(2+)-activated chloride channel. May play a role in Ca(2+) signal transduction. May be involved in cell proliferation and cell aggregation (By similarity). Publication Abstract from PubMedTweety homologs (TTYHs) comprise a conserved family of transmembrane proteins found in eukaryotes with three members (TTYH1-3) in vertebrates. They are widely expressed in mammals including at high levels in the nervous system and have been implicated in cancers and other diseases including epilepsy, chronic pain, and viral infections. TTYHs have been reported to form Ca(2+)- and cell volume-regulated anion channels structurally distinct from any characterized protein family with potential roles in cell adhesion, migration, and developmental signaling. To provide insight into TTYH family structure and function, we determined cryo-EM structures of Mus musculus TTYH2 and TTYH3 in lipid nanodiscs. TTYH2 and TTYH3 adopt a previously unobserved fold which includes an extended extracellular domain with a partially solvent exposed pocket that may be an interaction site for hydrophobic molecules. In the presence of Ca(2+), TTYH2 and TTYH3 form homomeric cis-dimers bridged by extracellularly coordinated Ca(2+). Strikingly, in the absence of Ca(2+), TTYH2 forms trans-dimers that span opposing membranes across a ~130 A intermembrane space as well as a monomeric state. All TTYH structures lack ion conducting pathways and we do not observe TTYH2-dependent channel activity in cells. We conclude TTYHs are not pore forming subunits of anion channels and their function may involve Ca(2+)-dependent changes in quaternary structure, interactions with hydrophobic molecules near the extracellular membrane surface, and/or association with additional protein partners. Structures of tweety homolog proteins TTYH2 and TTYH3 reveal a Ca(2+)-dependent switch from intra- to intermembrane dimerization.,Li B, Hoel CM, Brohawn SG Nat Commun. 2021 Nov 25;12(1):6913. doi: 10.1038/s41467-021-27283-8. PMID:34824283[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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