RmlC: Difference between revisions

Latest revision as of 10:59, 6 March 2022

Function

RmlC or dTDP-4-dehydrorhamnose 3,5-epimerase or dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase is an epimerase enzyme of the dTPD-rhamnose pathway. Deoxy-thymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose. L-rhamnose is a sugar component of microbial cell wall and is absent in human^[1].

Relevance

Inhibitors of RmlC can serve as potential antimicrobial drugs^[2].

Structural highlights

The ^[3]. Water molecules are shown as red spheres.

3D structures of RmlC3D structures of RmlC

Updated on 06-March-2022

ReferencesReferences

↑ Giraud MF, Leonard GA, Field RA, Berlind C, Naismith JH. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase. Nat Struct Biol. 2000 May;7(5):398-402. PMID:10802738 doi:10.1038/75178
↑ Kantardjieff KA, Kim CY, Naranjo C, Waldo GS, Lekin T, Segelke BW, Zemla A, Park MS, Terwilliger TC, Rupp B. Mycobacterium tuberculosis RmlC epimerase (Rv3465): a promising drug-target structure in the rhamnose pathway. Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):895-902. Epub 2004, Apr 21. PMID:15103135 doi:10.1107/S0907444904005323
↑ Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH. RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787 doi:http://dx.doi.org/10.1016/j.jmb.2006.09.063

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Giraud MF, Leonard GA, Field RA, Berlind C, Naismith JH. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase. Nat Struct Biol. 2000 May;7(5):398-402. PMID:10802738 doi:10.1038/75178

[2] Kantardjieff KA, Kim CY, Naranjo C, Waldo GS, Lekin T, Segelke BW, Zemla A, Park MS, Terwilliger TC, Rupp B. Mycobacterium tuberculosis RmlC epimerase (Rv3465): a promising drug-target structure in the rhamnose pathway. Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):895-902. Epub 2004, Apr 21. PMID:15103135 doi:10.1107/S0907444904005323

[3] Dong C, Major LL, Srikannathasan V, Errey JC, Giraud MF, Lam JS, Graninger M, Messner P, McNeil MR, Field RA, Whitfield C, Naismith JH. RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation. J Mol Biol. 2007 Jan 5;365(1):146-59. Epub 2006 Sep 29. PMID:17046787 doi:http://dx.doi.org/10.1016/j.jmb.2006.09.063

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@@ Line 1: / Line 1: @@
-<StructureSection load='2ixc' size='340' side='right' caption='Structure of RmlC complex with dTPD-rhamnose (PDB code [[2ixc]]).' scene=''>
+<StructureSection load='2ixc' size='350' side='right' caption='Structure of RmlC complex with dTPD-rhamnose (PDB code [[2ixc]]).' scene='59/597941/Cv/1'>
-'''RmlC''' is an enzyme of the dTPD-rhamnose pathway.  Deoxy-thymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose.  L-rhamnose is a sugar component of microbial cell wall and is absent in human.
 == Function ==
+'''RmlC''' or '''dTDP-4-dehydrorhamnose 3,5-epimerase''' or '''dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase''' is an epimerase enzyme of the dTPD-rhamnose pathway.  Deoxy-thymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose.  L-rhamnose is a sugar component of microbial cell wall and is absent in human<ref>PMID:10802738</ref>.
-== Disease ==
 == Relevance ==
+Inhibitors of RmlC can serve as potential antimicrobial drugs<ref>PMID:15103135</ref>.
-Inhibitors of RmlC can serve as potential antimicrobial drugs.
 == Structural highlights ==
+The <scene name='59/597941/Cv/4'>active site of RmlC contains the reaction product DTDP-L-rhamnose</scene><ref>PMID:17046787</ref>. Water molecules are shown as red spheres.
+</StructureSection>
 ==3D structures of RmlC==
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
-===RmlC===
+*RmlC
-[[1pm7]], [[1upi]] – MtRmlC – ''Mycobacterium tuberculosis''<br />
-[[2ixj]], [[1rtv]] – PaRmlC – ''Pseudomonas aeruginosa''<br />
-[[1nxm]] – SsRmlC – ''Streptococcus suis''<br />
-[[1ep0]] – MetRmlC – ''Methanothermobacter thermautotrophicus''<br />
-[[1dzr]] – StRmlC – ''Salmonella typhimurium''<br />
-===RmlC complexes===
+**[[1pm7]], [[1upi]] – MtRmlC – ''Mycobacterium tuberculosis''<br />
+**[[2ixj]], [[1rtv]] – PaRmlC – ''Pseudomonas aeruginosa''<br />
+**[[1nxm]] – SsRmlC – ''Streptococcus suis''<br />
+**[[1ep0]] – MetRmlC – ''Methanothermobacter thermautotrophicus''<br />
+**[[1dzr]] – StRmlC – ''Salmonella typhimurium''<br />
-[[2ixc]] – MtRmlC + dTDP rhamnose <br />
+*RmlC complexes
-[[2ixh]] – PaRmlC + dTDP rhamnose <br />
-[[2ixl]] – SsRmlC + dTDP rhamnose <br />
-[[2ixk]] – PaRmlC + dTDP-keto rhamnose <br />
-[[1nyw]] – SsRmlC + dTDP glucose <br />
-[[1nzc]] – SsRmlC + dTDP xylose <br />
-[[1epz]] – MetRmlC + TDP <br />
-[[3ryk]] – RmlC + TDP + pyrophosphate – ''Bacillus anthracis''<br />
-[[1dzt]] – StRmlC + acetylthymidine diphosphate<br />
+**[[2ixc]] – MtRmlC + dTDP rhamnose <br />
+**[[2ixh]] – PaRmlC + dTDP rhamnose <br />
+**[[2ixl]] – SsRmlC + dTDP rhamnose <br />
+**[[2ixk]] – PaRmlC + dTDP-keto rhamnose <br />
+**[[6ndr]] – RmlC + dTDP-keto rhamnose – ''Legionella pneumophila''<br />
+**[[1nyw]] – SsRmlC + dTDP glucose <br />
+**[[1nzc]] – SsRmlC + dTDP xylose <br />
+**[[1epz]] – MetRmlC + TDP <br />
+**[[3ryk]] – RmlC + TDP + pyrophosphate – ''Bacillus anthracis''<br />
+**[[1dzt]] – StRmlC + acetylthymidine diphosphate<br />
+}}
 == References ==
 <references/>
 [[Category:Topic Page]]