Barnase: Difference between revisions

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<StructureSection load='1brn' size='450' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>


== Function ==
== Function ==


'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. <ref>PMID:8110767</ref>
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.  


== Relevance ==
== Relevance ==
Line 11: Line 11:
== Structural highlights ==
== Structural highlights ==


BA hydrolyzes RNA at GpN sites where the active site positively charged Lys and Arg coordinate the RNA phosphate group.
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
 
</StructureSection>


== 3D Structures of Barnase ==
== 3D Structures of Barnase ==
[[Barnase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*Barnase
 
**[[3kch]], [[2f4y]] – BA cross-linked<br />
**[[2f56]] - BA cross-linked + urea<br />
**[[2f5w]] - BA cross-linked + thiourea<br />
**[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
**[[1fw7]], [[1bnr]] – BA - NMR<br />
**[[2kf3]] – BA (mutant) – NMR<br />
**[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
**[[1yvs]] – BA domain swapped<br />
**[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
**[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
 
*Barnase + BST
 
**[[3da7]] - BA (mutant) + BST<br />
**[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
**[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
**[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />


**[[2c4b]] – BA/MCOEETI (mutant)
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 17:37, 20 February 2022


Function

Barnase (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. [1] The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.

Relevance

BA structure was used extensively for studying protein folding.

Structural highlights

BA hydrolyzes RNA at GpN sites where the (Lys and Arg are in magenta).

3D Structures of Barnase

Barnase 3D structures


Barnase complex with tetranucleotide (PDB code 1brn)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Buckle AM, Fersht AR. Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Biochemistry. 1994 Feb 22;33(7):1644-53. PMID:8110767

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Jaime Prilusky, Alexander Berchansky
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