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| {{STRUCTURE_1bni| PDB=1bni | SIZE=400| SCENE=Barnase/Cv/1 |right|CAPTION=3 monomers of barnase [[1bni]] }}
| | <StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'> |
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| '''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
| | == Function == |
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| __NOTOC__
| | '''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA. |
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| == 3D Structures of Barnase == | | == Relevance == |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | BA structure was used extensively for studying protein folding. |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Barnase
| | == Structural highlights == |
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| **[[3kch]], [[2f4y]] – BA cross-linked<br />
| | BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta). |
| **[[2f56]] - BA cross-linked + urea<br />
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| **[[2f5w]] - BA cross-linked + thiourea<br />
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| **[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
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| **[[1fw7]], [[1bnr]] – BA - NMR<br />
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| **[[2kf3]] – BA (mutant) – NMR<br />
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| **[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
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| **[[1yvs]] – BA domain swapped<br />
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| **[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
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| **[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide
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| *Barnase + BST
| | == 3D Structures of Barnase == |
| | [[Barnase 3D structures]] |
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| **[[3da7]] - BA (mutant) + BST<br />
| | </StructureSection> |
| **[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
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| **[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
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| **[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />
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| **[[2c4b]] – BA/MCOEETI (mutant)
| | == References == |
| }}
| | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |