Barnase: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(24 intermediate revisions by 2 users not shown)
Line 1: Line 1:
[[Image:1bni.png|left|200px|thumb|Crystal Structure of Barnase [[1bni]]]]
<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>
{{STRUCTURE_1bni|  PDB=1bni  | SIZE=300| SCENE=Barnase/Cv/1 |right|CAPTION=Barnase [[1bni]] }}


'''Barnase''' (BA, 'BActerial RiboNucleASE') is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''.  The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
== Function ==


{{TOC limit|limit=2}}
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.


== 3D Structures of Barnase ==
== Relevance ==
 
BA structure was used extensively for studying protein folding.


[[3kch]], [[2f4y]] – BA cross-linked<br />
== Structural highlights ==
[[2f56]] - BA cross-linked + urea<br />
[[2f5w]] - BA cross-linked + thiourea<br />
[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
[[1fw7]], [[1bnr]] – BA - NMR<br />
[[2kf3]] – BA (mutant) – NMR<br />
[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
[[1yvs]] – BA domain swapped<br />
[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide


===BA + BST===
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).


[[3da7]] - BA (mutant) + BST<br />
== 3D Structures of Barnase ==
[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
[[Barnase 3D structures]]
[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />


[[2c4b]] – BA/MCOEETI (mutant)
</StructureSection>


== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 17:37, 20 February 2022


Function

Barnase (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. [1] The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.

Relevance

BA structure was used extensively for studying protein folding.

Structural highlights

BA hydrolyzes RNA at GpN sites where the (Lys and Arg are in magenta).

3D Structures of Barnase

Barnase 3D structures


Barnase complex with tetranucleotide (PDB code 1brn)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Buckle AM, Fersht AR. Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution. Biochemistry. 1994 Feb 22;33(7):1644-53. PMID:8110767

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Jaime Prilusky, Alexander Berchansky
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=Barnase&oldid=3519088"