Purine repressor: Difference between revisions
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<StructureSection load=' | <StructureSection load='' size='450' side='right' caption='Structure of E. coli purine processor complex with DNA and guanine (PDB entry [[1wet]])' scene='55/554904/Cv/1'> | ||
== Function == | == Function == | ||
'''Purine repressor''' (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>. | '''Purine repressor''' (PurR) is a member of the lac repressor family. PurP binds DNA via a highly conserved helix-turn-helix at the N terminal (DBD). PurP contains a nucleotide co-repressor binding domain as well (CBD). PurP binds to a 16-bp operator sequence and co-regulates genes which are involved in the biosynthesis of purine and pyrimidine nucleotides<ref>PMID:1971621</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
The PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones. | The <scene name='55/554904/Cv/5'>PurP guanine co-repressor binding site includes stacking interactions as well as hydrogen bonded ones</scene>. Water molecules are shown as red spheres. The DNA binding domain contains a <scene name='55/554904/Cv/6'>helix-turn-helix-loop-helix motif which interacts with the DNA major groove</scene> and a hinge helix binding to to the DNA minor groove. <scene name='55/554904/Cv/7'>Residue L54 interdigitates with the DNA central base pair</scene><ref>PMID:9278422</ref>. | ||
</StructureSection> | </StructureSection> | ||
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**[[1dbq]] - EcPurR CBD + Mg - NMR<br /> | **[[1dbq]] - EcPurR CBD + Mg - NMR<br /> | ||
**[[1jhz]] - EcPurR CBD (mutant) + Mg <br /> | **[[1jhz]] - EcPurR CBD (mutant) + Mg <br /> | ||
**[[1o57]] - BsPurR - ''Bacillus subtilis''<br /> | |||
*Purine repressor complex with DNA and nucleotide | *Purine repressor complex with DNA and nucleotide | ||
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**[[2puc]], [[2puf]] - EcPurR (mutant) + DNA + guanine <br /> | **[[2puc]], [[2puf]] - EcPurR (mutant) + DNA + guanine <br /> | ||
**[[1zay]] - EcPurR + DNA + hypoxanthine + di-imino-purine<br /> | **[[1zay]] - EcPurR + DNA + hypoxanthine + di-imino-purine<br /> | ||
**[[7rmw]] – BsPurR + PPGPP<br /> | |||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||