Protein kinase Spk1: Difference between revisions
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<StructureSection load=' | <StructureSection load='' size='350' side='right' caption='Structure of yeast Rad53 FHA1 domain (cyan) complex with phosphothreonine peptide (green) (PDB code [[1k3n]]).' scene='59/590826/Cv/1'> | ||
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== Structural highlights == | == Structural highlights == | ||
Rad53 contains phosphothreonine (PTO) recognition domains: '''FHA1''' at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to PTO and '''FHA2''' at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to PTO. Two '''SCD''' - SQ/TQ-rich cluster domains – are flanking the '''kinase''' domain. SCD domain is associated with DNA-damage-response proteins. The <scene name='59/590826/Cv/ | Rad53 contains phosphothreonine (PTO) recognition domains: '''FHA1''' at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to PTO and '''FHA2''' at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to PTO. Two '''SCD''' - SQ/TQ-rich cluster domains – are flanking the '''kinase''' domain. SCD domain is associated with DNA-damage-response proteins. The <scene name='59/590826/Cv/4'>yeast FHA1 domain interacts with peptide containing PTOXXD sequence</scene><ref>PMID:11846567</ref>. | ||
</StructureSection> | </StructureSection> | ||
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{{#tree:id=OrganizedByTopic|openlevels=0| | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
*Rad53 FHA1 domain | *Rad53 FHA1 domain 1-164 | ||
**[[1k3j]] – yRad53 – yeast - NMR<br /> | **[[1k3j]] – yRad53 – yeast - NMR<br /> | ||
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**[[5t2f]] - yRad53/DBF4 BRCT domain<br /> | **[[5t2f]] - yRad53/DBF4 BRCT domain<br /> | ||
**[[5t2s]] - yRad53/DBF4 BRCT domain + phosphopeptide<br /> | **[[5t2s]] - yRad53/DBF4 BRCT domain + phosphopeptide<br /> | ||
*Rad53 FHA2 domain | |||
*Rad53 FHA2 domain 573-730 | |||
**[[1qu5]], [[1dmz]], [[1fhq]] – yRad53 – yeast - NMR<br /> | **[[1qu5]], [[1dmz]], [[1fhq]] – yRad53 – yeast - NMR<br /> | ||
**[[1fhr]], [[1j4k]], [[1j4l]], [[1k2m]], [[1k2n]] – yRad53 + Rad9 peptide - NMR<br /> | **[[1fhr]], [[1j4k]], [[1j4l]], [[1k2m]], [[1k2n]] – yRad53 + Rad9 peptide - NMR<br /> | ||
*Rad53 kinase and SCD2 domains | *Rad53 kinase and SCD2 domains residues 170-512 | ||
**[[4pdp]] – yRad53 kinase and SCD2 domain (mutant)<br /> | **[[4pdp]] – yRad53 kinase and SCD2 domain (mutant)<br /> | ||
**[[4pds]] – yRad53 kinase and SCD2 domain (mutant) + AMPPNP<br /> | **[[4pds]] – yRad53 kinase and SCD2 domain (mutant) + AMPPNP<br /> | ||
*Rad53 residues 1-466 | |||
**[[5xzw]] – yRad53 <br /> | |||
**[[5xzv]] – yRad53 + AMPPNP<br /> | |||
}} | }} | ||