Protein kinase Spk1: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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-<StructureSection load='1k3n' size='340' side='right' caption='Structure of yeast Rad53 FHA1 domain (grey) complex with phosphothreonine peptide (green) (PDB code [[1k3n]]).' scene=''>
+<StructureSection load='' size='350' side='right' caption='Structure of yeast Rad53 FHA1 domain (cyan) complex with phosphothreonine peptide (green) (PDB code [[1k3n]]).' scene='59/590826/Cv/1'>
 == Function ==
-'''Protein kinase Spk1 (Rad53)''' is a yeast serine/threonine protein kinase which phosphorylates proteins on serene, threonine and tyrosine<ref>PMID:1899289</ref>.  Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints.  Rad53 phosphorylates proteins at serine, threonine and tyrosine residues.
+'''Protein kinase Spk1 (Rad53)''' is a yeast serine/threonine protein kinase which phosphorylates proteins on serine, threonine and tyrosine<ref>PMID:1899289</ref>.  Rad53 controls S-phase checkpoint and G1 and G2 DNA damage checkpoints.
 == Structural highlights ==
-Rad53 contains phosphothreonine recognition domains: '''FHA1''' at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to phosphothreonine and '''FHA2''' at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to phosphothreonine. Two '''SCD''' - SQ/TQ-rich cluster domains – are flanking the '''kinase''' domain. SCD domain is associated with DNA-damage-response proteins. The yeast FHA1 domain interacts with peptide containing phosphothreonine<ref>PMID:11106755</ref>.
+Rad53 contains phosphothreonine (PTO) recognition domains: '''FHA1''' at the N-terminal (residues 1-164) which selects for Asp at position +3 relative to PTO and '''FHA2''' at the C-terminal (residues 573-730) which selects for Ile at position +3 relative to PTO. Two '''SCD''' - SQ/TQ-rich cluster domains – are flanking the '''kinase''' domain. SCD domain is associated with DNA-damage-response proteins. The <scene name='59/590826/Cv/4'>yeast FHA1 domain interacts with peptide containing PTOXXD sequence</scene><ref>PMID:11846567</ref>.
 </StructureSection>
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 {{#tree:id=OrganizedByTopic|openlevels=0|
-*Rad53 FHA1 domain
+*Rad53 FHA1 domain 1-164
-**[[1k3j]] – yRad53 FHA1 domain – yeast - NMR<br />
+**[[1k3j]] – yRad53 – yeast - NMR<br />
-**[[1g3g]], [[1j4o]] – yRad53 FHA1 domain (mutant) - NMR<br />
+**[[1g3g]], [[1j4o]] – yRad53 (mutant) - NMR<br />
-**[[1j4p]], [[1j4q]], [[1k3n]], [[1k3q]] – yRad53 FHA1 domain + Rad9 peptide - NMR<br />
+**[[1j4p]], [[1j4q]], [[1k3n]], [[1k3q]] – yRad53 + Rad9 peptide - NMR<br />
-**[[1g6g]], [[2a0t]] – yRad53 FHA1 domain + phosphothreonine peptide <br />
+**[[1g6g]], [[2a0t]] – yRad53 + PTO peptide <br />
-**[[2jqi]] – yRad53 FHA1 domain + phosphothreonine peptide - NMR<br />
+**[[2jqi]] – yRad53 + PTO peptide - NMR<br />
+**[[5t2f]] - yRad53/DBF4 BRCT domain<br />
+**[[5t2s]] - yRad53/DBF4 BRCT domain + phosphopeptide<br />
-*Rad53 FHA2 domain
+*Rad53 FHA2 domain 573-730
-**[[1qu5]], [[1dmz]], [[1fhq]] – yRad53 FHA2 domain – yeast - NMR<br />
+**[[1qu5]], [[1dmz]], [[1fhq]] – yRad53 – yeast - NMR<br />
-**[[1fhr]], [[1j4k]], [[1j4l]], [[1k2m]], [[1k2n]] – yRad53 FHA2 domain + Rad9 peptide - NMR<br />
+**[[1fhr]], [[1j4k]], [[1j4l]], [[1k2m]], [[1k2n]] – yRad53 + Rad9 peptide - NMR<br />
-*Rad53 kinase and SCD2 domains
+*Rad53 kinase and SCD2 domains residues 170-512
 **[[4pdp]] – yRad53 kinase and SCD2 domain (mutant)<br />
 **[[4pds]] – yRad53 kinase and SCD2 domain (mutant) + AMPPNP<br />
+*Rad53 residues 1-466
+**[[5xzw]] – yRad53  <br />
+**[[5xzv]] – yRad53 + AMPPNP<br />
 }}