2kgh: Difference between revisions
New page: '''Unreleased structure''' The entry 2kgh is ON HOLD Authors: Corzo, G., Bernard, C., Clement, H., Bosmans, F., Tygat, J., Possani, L.D., Darbon, H., Alagon, A. Description: Solution s... |
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The | ==Solution structure of Brachyperma ruhnaui toxin 2== | ||
<StructureSection load='2kgh' size='340' side='right'caption='[[2kgh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kgh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brachypelma_ruhnaui Brachypelma ruhnaui]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KGH FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgh OCA], [https://pdbe.org/2kgh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kgh RCSB], [https://www.ebi.ac.uk/pdbsum/2kgh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kgh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[https://www.uniprot.org/uniprot/TXP2_BRARH TXP2_BRARH]] Has insecticidal activity.[REFERENCE:1] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kgh_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kgh ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Soluble venom and purified fractions of the theraposid spider Brachypelma albiceps were screened for insecticidal peptides based on toxicity to crickets. Two insecticidal peptides, named Ba1 and Ba2, were obtained after the soluble venom was separated by high performance liquid chromatography and cation exchange chromatography. The two insecticidal peptides contain 39 amino acid residues and three disulfide bonds, and based on their amino acid sequence, they are highly identical to the insecticidal peptides from the theraposid spiders Aphonopelma sp. from the USA and Haplopelma huwenum from China indicating a relationship among these genera. Although Ba1 and Ba2 were not able to modify currents in insect and vertebrate cloned voltage-gated sodium ion channels, they have noteworthy insecticidal activities compared to classical arachnid insecticidal toxins indicating that they might target unknown receptors in insect species. The most abundant insecticidal peptide Ba2 was submitted to NMR spectroscopy to determine its 3-D structure; a remarkable characteristic of Ba2 is a cluster of basic residues, which might be important for receptor recognition. | |||
Insecticidal peptides from the theraposid spider Brachypelma albiceps: an NMR-based model of Ba2.,Corzo G, Bernard C, Clement H, Villegas E, Bosmans F, Tytgat J, Possani LD, Darbon H, Alagon A Biochim Biophys Acta. 2009 Aug;1794(8):1190-6. Epub 2009 Apr 15. PMID:19374957<ref>PMID:19374957</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2kgh" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Brachypelma ruhnaui]] | |||
[[Category: Large Structures]] | |||
[[Category: Alagon, A]] | |||
[[Category: Bernard, C]] | |||
[[Category: Bosmans, F]] | |||
[[Category: Clement, H]] | |||
[[Category: Corzo, G]] | |||
[[Category: Darbon, H]] | |||
[[Category: Possani, L D]] | |||
[[Category: Tygat, J]] | |||
[[Category: Disulfide bond]] | |||
[[Category: Insecticidal peptide]] | |||
[[Category: Neurotoxin]] | |||
[[Category: Secreted]] | |||
[[Category: Toxin]] | |||