CRISPR-Cas9: Difference between revisions
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<StructureSection load='' size='350' side='right' scene='74/742625/Cv3/ | <StructureSection load='' size='350' side='right' scene='74/742625/Cv3/13' caption='Cas9 complex with RNA (PDB code [[4zt0]])'> | ||
'''Part I''' | '''Part I''' | ||
SEE ALSO [[CRISPR-Cas]] | SEE ALSO | ||
*[[CRISPR-Cas9 Part II]] | |||
*[[CRISPR-Cas]] | |||
'''Background''' | '''Background''' | ||
Highlights | Highlights | ||
*'''CRISPR-Cas9''' is a powerful tool to modulate transcription in wide range of cell types. | *'''CRISPR-Cas9 (CRISPR type II-A)''' is a powerful tool to modulate transcription in wide range of cell types. | ||
*An expanding set of CRISPR-based transcription effectors is available. | *An expanding set of CRISPR-based transcription effectors is available. | ||
*Gene networks can be efficiently probed and modified for biotechnology applications.<ref name="Did">PMID:27344519</ref> | *Gene networks can be efficiently probed and modified for biotechnology applications.<ref name="Did">PMID:27344519</ref> | ||
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'''Cas9-sgRNA-target DNA complexes from Streptococcus pyogenes:''' | '''Cas9-sgRNA-target DNA complexes from Streptococcus pyogenes:''' | ||
*<scene name='74/746096/Cv3/1'>Domain organization of nuclease lobe of Cas9 from S. pyogenes</scene> | *<scene name='74/746096/Cv3/1'>Domain organization of nuclease lobe of Cas9 from S. pyogenes</scene> ([[4zt0]]) | ||
*<scene name='74/742625/Cv/42'>Cas9-sgRNA-target DNA complex from Streptococcus pyogenes</scene> ([[5fw2]]). | *<scene name='74/742625/Cv/42'>Cas9-sgRNA-target DNA complex from Streptococcus pyogenes</scene> ([[5fw2]]). | ||
*<scene name='74/742625/Cv2/12'>Cas9-sgRNA-target DNA complex from Streptococcus pyogenes</scene> ([[5b2s]]). | *<scene name='74/742625/Cv2/12'>Cas9-sgRNA-target DNA complex from Streptococcus pyogenes</scene> ([[5b2s]]). | ||
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Other representatives: [[5y36]], [[4un3]]. | Other representatives: [[5y36]], [[4un3]]. | ||
'''For continuation please see [[CRISPR-Cas9 Part II]]''' | |||
=See aslo= | =See aslo= | ||
*[[Cas9]] | *[[Cas9]] | ||
*[[Endonuclease]] | *[[Endonuclease]] | ||
*[[Cas9 (hebrew)]] | |||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||