2zds: Difference between revisions
Jump to navigation
Jump to search
New page: '''Unreleased structure''' The entry 2zds is ON HOLD until Nov 27 2009 Authors: Begum, P., Sakai, N., Hayashi, T., Tamura, T., Watanabe, N., Yao, M., Tanaka, I. Description: Crystal St... |
No edit summary |
||
| (8 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)== | ||
<StructureSection load='2zds' size='340' side='right'caption='[[2zds]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2zds]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZDS FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sco6571 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zds OCA], [https://pdbe.org/2zds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zds RCSB], [https://www.ebi.ac.uk/pdbsum/2zds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zds ProSAT]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zd/2zds_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zds ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
SCO6571 protein from Streptomyces coelicolor A3(2) was overexpressed and purified using Rhodococcus erythropolis as an expressing host. Crystals of selenomethionine-substituted SCO6571 have been obtained by vapor diffusion method. SCO6571 crystals diffract to 2.3 A and were found to belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell parameters a = 84.5, b = 171.6, c = 184.8 A. Six molecules in the asymmetric unit give a crystal volume per protein mass (V(M)) of 2.97 A (3) Da(-1) and solvent content of 58.6 %. The structure was solved by the single wavelength anomalous diffraction (SAD) method. SCO6571 is a TIM-barrel fold protein that assembles into a hexameric molecule with D(3) symmetry. | |||
Crystal structure of SCO6571 from Streptomyces coelicolor A3(2).,Begum P, Sakai N, Hayashi T, Gao YG, Tamura T, Watanabe N, Yao M, Tanaka I Protein Pept Lett. 2008;15(7):709-12. PMID:18782066<ref>PMID:18782066</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2zds" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Begum, P]] | |||
[[Category: Gao, Y G]] | |||
[[Category: Sakai, N]] | |||
[[Category: Tanaka, I]] | |||
[[Category: Watanabe, N]] | |||
[[Category: Yao, M]] | |||
[[Category: Dna binding protein]] | |||
[[Category: Dna-binding]] | |||
[[Category: National project on protein structural and functional analyse]] | |||
[[Category: Nppsfa]] | |||
[[Category: Structural genomic]] | |||
[[Category: Tim-barrel fold]] | |||
Latest revision as of 13:50, 8 December 2021
Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSCO6571 protein from Streptomyces coelicolor A3(2) was overexpressed and purified using Rhodococcus erythropolis as an expressing host. Crystals of selenomethionine-substituted SCO6571 have been obtained by vapor diffusion method. SCO6571 crystals diffract to 2.3 A and were found to belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell parameters a = 84.5, b = 171.6, c = 184.8 A. Six molecules in the asymmetric unit give a crystal volume per protein mass (V(M)) of 2.97 A (3) Da(-1) and solvent content of 58.6 %. The structure was solved by the single wavelength anomalous diffraction (SAD) method. SCO6571 is a TIM-barrel fold protein that assembles into a hexameric molecule with D(3) symmetry. Crystal structure of SCO6571 from Streptomyces coelicolor A3(2).,Begum P, Sakai N, Hayashi T, Gao YG, Tamura T, Watanabe N, Yao M, Tanaka I Protein Pept Lett. 2008;15(7):709-12. PMID:18782066[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
| ||||||||||||||||||