Electron cryomicroscopy: Difference between revisions
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===Visualizing EM Maps=== | ===Visualizing EM Maps=== | ||
[http://firstglance.jmol.org FirstGlance in Jmol] makes it easy to visualize EM density maps, as shown in the example at right. Load your [[PDB ID]] (or [[6nef]]). Then, use '''Find..''' (in the [https://proteopedia.org/wiki/fgij/where.htm#focusbox Focus Box]) to locate the residues of interest (or "503,169,360" for 6nef -- In 6nef, these happen to be unambiguous sequence numbers for HEC503, HIS169, and HIS360.) Click on "EM Density Map". After adjusting the view as you wish, click "Save Image or Animation for Powerpoint". An example of an animation saved from FirstGlance is above in this page. | [http://firstglance.jmol.org FirstGlance in Jmol] makes it easy to visualize EM density maps, as shown in the example at right. Load your [[PDB ID]] (or [[6nef]]). Then, use '''Find..''' (in the [https://proteopedia.org/wiki/fgij/where.htm#focusbox Focus Box]) to locate the residues of interest (or "503,169,360" for 6nef -- In 6nef, these happen to be unambiguous sequence numbers for HEC503, HIS169, and HIS360.) Click on "EM Density Map". After adjusting the view as you wish, click "Save Image or Animation for Powerpoint". An example of an animation saved from FirstGlance is above in this page. | ||
{{Template:PDBMapViewers}} | |||
===The Electron Microscopy Data Bank=== | ===The Electron Microscopy Data Bank=== | ||
The [[Electron Microscopy Data Bank]] (EMDB) archives EM density maps. Only "all features" maps are deposited, as cryo-EM analysis has no equivalent to the [[Electron_density_maps#Fo-Fc_Difference_Map|difference map]] of X-ray diffraction. | The [[Electron Microscopy Data Bank]] (EMDB) archives EM density maps. Only "all features" maps are deposited, as '''cryo-EM analysis has no equivalent to the [[Electron_density_maps#Fo-Fc_Difference_Map|difference map]]''' of X-ray diffraction. | ||
==Temperatures (B factors)== | ==Temperatures (B factors)== | ||