2ads: Difference between revisions
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{{Theoretical_model}} | |||
==THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.== | ==THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.== | ||
<StructureSection load='2ads' size='340' side='right' caption='[[2ads]]' scene=''> | <StructureSection load='2ads' size='340' side='right'caption='[[2ads]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ADS FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ads FirstGlance], [https://www.ebi.ac.uk/pdbsum/2ads PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ads ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Theoretical Model]] | |||
[[Category: Large Structures]] | |||
[[Category: Cheng, H Y]] | [[Category: Cheng, H Y]] | ||
[[Category: Dauter, Z]] | [[Category: Dauter, Z]] | ||
Latest revision as of 17:39, 17 November 2021
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THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.
Structural highlights
Publication Abstract from PubMedArgonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC. Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage.,Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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