NAD synthase: Difference between revisions

Latest revision as of 12:58, 15 November 2021

Function

NAD+ synthase or NH3-dependent NAD synthetase (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH₃ to AMP, NAD+ and diphosphate (PP). NADS is part of the NAD synthesis pathway^[1] . See NAD.

Structural highlights

The contains . The ^[2]. Water molecules are shown as red spheres.

NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry 1xng)

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3D Structures of NAD+ synthase3D Structures of NAD+ synthase

Updated on 15-November-2021

ReferencesReferences

↑ Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD(+) synthetase that is functionally conserved in mammals. Yeast. 2003 Aug;20(11):995-1005. PMID:12898714 doi:http://dx.doi.org/10.1002/yea.1008
↑ Kang GB, Kim YS, Im YJ, Rho SH, Lee JH, Eom SH. Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori. Proteins. 2005 Mar 1;58(4):985-8. PMID:15645437 doi:10.1002/prot.20377

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

[1] Suda Y, Tachikawa H, Yokota A, Nakanishi H, Yamashita N, Miura Y, Takahashi N. Saccharomyces cerevisiae QNS1 codes for NAD(+) synthetase that is functionally conserved in mammals. Yeast. 2003 Aug;20(11):995-1005. PMID:12898714 doi:http://dx.doi.org/10.1002/yea.1008

[2] Kang GB, Kim YS, Im YJ, Rho SH, Lee JH, Eom SH. Crystal structure of NH3-dependent NAD+ synthetase from Helicobacter pylori. Proteins. 2005 Mar 1;58(4):985-8. PMID:15645437 doi:10.1002/prot.20377

[1]

[2]

@@ Line 1: / Line 1: @@
-<StructureSection load='1xng' size='350' side='right' caption='NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry [[1xng]])' scene='54/541072/Cv/1'>
+<StructureSection load='' size='350' side='right' caption='NAD+ synthase complex with Mg+2 (green), ATP and deamino-NAD (PDB entry [[1xng]])' scene='54/541072/Cv/1'>
 == Function ==
-'''NAD+ synthase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH<sub>3</sub> to AMP, NAD+ and diphosphate (PP).  NADS is part of the NAD synthesis pathway<ref>PMID:12898714</ref> .  See [[NAD]].
+'''NAD+ synthase''' or '''NH3-dependent NAD synthetase''' (NADS) catalyzes the conversion of ATP, deamino-NAD+ and NH<sub>3</sub> to AMP, NAD+ and diphosphate (PP).  NADS is part of the NAD synthesis pathway<ref>PMID:12898714</ref> .  See [[NAD]].
 == Structural highlights ==
-The <scene name='54/541072/Cv/4'>ATP binding site</scene> contains <scene name='54/541072/Cv/5'>Mg+2 ion</scene>.  The <scene name='54/541072/Cv/7'>NAD binding site is located in the interface of the 2 subunits</scene><ref>PMID:15645437</ref>. Water molecules shown as red spheres.
+The <scene name='54/541072/Cv/8'>ATP binding site</scene> contains <scene name='54/541072/Cv/9'>Mg+2 ion</scene>. The <scene name='54/541072/Cv/10'>NAD binding site is located in the interface of the 2 subunits</scene><ref>PMID:15645437</ref>. Water molecules are shown as red spheres.
 </StructureSection>
 == 3D Structures of NAD+ synthase ==
@@ Line 20: / Line 20: @@
 **[[2pzb]] – BaNADS  - ''Bacillus anthracis'' <br />
 **[[3dpi]] – NADS – ''Burkholderia pseudomallei''<br />
+**[[6kv3]] – NADS – ''Staphylococcus aureus''<br />
+**[[5wp0]] – NADS – ''Allivibrio fischeri''<br />
+**[[5huh]], [[5huj]] – NADS – ''Streptococcus pyogenes''<br />
+**[[3n05]] – NADS – ''Streptomyces avermitillis''<br />
+**[[4xfd]] – PaNADS – ''Pseudomonas aeruginosa''<br />
+**[[4q16]] – NADS – ''Deinococcus radiodurans''<br />
+**[[2e18]] – NADS – ''Pyrococcus horikoshii''<br />
 *NADS binary complex
@@ Line 26: / Line 33: @@
 **[[1wxh]] – EcNADS + NAD  <br />
 **[[3hmq]] – BsNADS + NAD – ''Salmonella typhimurium'' <br />
+**[[6c8q]] – NADS + NAD – ''Enterococcus faecalis''<br />
+**[[5f23]] – PaNADS + NAD <br />
 *NADS ternary complex
@@ Line 33: / Line 42: @@
 **[[1wxg]] – EcNADS + Mg + deamino-NAD  <br />
 **[[2pz8]] – BaNADS + Mg + ATP derivative  <br />
+**[[3fiu]] – NADS + Mg + AMP – ''Francisella tularensis''<br />
 *NADS quaternary complex
@@ Line 50: / Line 60: @@
 *NADS glutamine-dependent
+**[[6ofb]] – hNADS + AMP + NAD derivative<br />
 **[[3sdb]] – MtNADS (mutant) – ''Mycobacterium tuberculosis'' <br />
 **[[3seq]], [[3sez]] – MtNADS (mutant) + ATP derivative + pyridinium derivative <br />
 **[[3szg]] – MtNADS (mutant) + AMP + pyridinium derivative <br />
 **[[3syt]] – MtNADS + AMP + NAD + glutamate <br />
+**[[3dla]] – MtNADS + norleucine + deamido-NAD <br />
+**[[6ofc]] – MtNADS (mutant) + AMP + sulfonamide derivative + glutamate <br />
+**[[4f4h]] – NADS – ''Burkholderia thailandensis''<br />
+**[[3ilv]] – NADS – ''Cytophaga hutchinsonii''<br />
 }}