2x8u: Difference between revisions

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-[[Image:2x8u.png|left|200px]]
-<!--
+==Sphingomonas wittichii Serine palmitoyltransferase==
-The line below this paragraph, containing "STRUCTURE_2x8u", creates the "Structure Box" on the page.
+<StructureSection load='2x8u' size='340' side='right'caption='[[2x8u]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2x8u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphww Sphww]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8U FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
--->
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Serine_C-palmitoyltransferase Serine C-palmitoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.50 2.3.1.50] </span></td></tr>
-{{STRUCTURE_2x8u|  PDB=2x8u  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8u OCA], [https://pdbe.org/2x8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8u RCSB], [https://www.ebi.ac.uk/pdbsum/2x8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8u ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8u_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8u ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, and some bacteria, and we have exploited the complete genome sequence of Sphingomonas wittichii to begin a complete analysis of bacterial sphingolipid biosynthesis. Here, we describe the enzymatic characterization of the SPT from this organism and present its high-resolution x-ray structure. Moreover, we identified an open reading frame with high sequence homology to acyl carrier proteins (ACPs) that are common to fatty acid biosynthetic pathways. This small protein was co-expressed with the SPT and we isolated and characterised the apo- and holo-forms of the ACP. Our studies suggest a link between fatty acid and sphingolipid metabolism. (c) 2010 Wiley Periodicals, Inc. Biopolymers 93: 811-822, 2010.
-===SPHINGOMONAS WITTICHII SERINE PALMITOYLTRANSFERASE===
+The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein.,Raman MC, Johnson KA, Clarke DJ, Naismith JH, Campopiano DJ Biopolymers. 2010 Sep;93(9):811-22. PMID:20578000<ref>PMID:20578000</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2x8u" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_20578000}}, adds the Publication Abstract to the page
+*[[Serine palmitoyltransferase|Serine palmitoyltransferase]]
-(as it appears on PubMed at http://www.pubmed.gov), where 20578000 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_20578000}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-[[2x8u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_wittichii Sphingomonas wittichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8U OCA].
-==Reference==
-<ref group="xtra">PMID:020578000</ref><references group="xtra"/>
 [[Category: Serine C-palmitoyltransferase]]
-[[Category: Sphingomonas wittichii]]
+[[Category: Sphww]]
-[[Category: Campopiano, D J.]]
+[[Category: Campopiano, D J]]
-[[Category: Johnson, K A.]]
+[[Category: Johnson, K A]]
-[[Category: Naismith, J H.]]
+[[Category: Naismith, J H]]
-[[Category: Raman, M C.C.]]
+[[Category: Raman, M C.C]]
 [[Category: Acyltransferase]]
 [[Category: Transferase]]