2tsb: Difference between revisions

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-{{Seed}}
-[[Image:2tsb.png|left|200px]]
-<!--
+==AZURIN MUTANT M121A-AZIDE==
-The line below this paragraph, containing "STRUCTURE_2tsb", creates the "Structure Box" on the page.
+<StructureSection load='2tsb' size='340' side='right'caption='[[2tsb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2tsb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tsb 1tsb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2TSB FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2tsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tsb OCA], [https://pdbe.org/2tsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2tsb RCSB], [https://www.ebi.ac.uk/pdbsum/2tsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2tsb ProSAT]</span></td></tr>
-{{STRUCTURE_2tsb|  PDB=2tsb  |  SCENE=  }}
+</table>
+== Function ==
+[[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ts/2tsb_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2tsb ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structures of the azurin mutant Met121Ala and its azide derivative Met121Ala-azide from Pseudomonas aeruginosa have been determined. The final crystallographic R values are 21.3 and 19.4% for the two structures, respectively. In the Met121Ala mutant, the distance between the copper ion and His117 increases by 0.34 A compared with the wild-type structure. The removal of the methionine in the apical position induces a shortening of the distance from the copper ion to the carbonyl O atom of Gly45 from 2.97 to 2.74 A. In the Met121Ala-azide structure, the azide anion occupies the cavity created by replacing the Met121 side chain with the smaller methyl group of Ala. The azide anion binds with a terminal N atom to the copper ion at a distance of about 2.04 A. In addition, the copper ion has moved out of the trigonal plane by about 0.26 A towards the azide anion. Thus, the copper site in this structure has a distorted tetrahedral arrangement. The spectroscopic characteristics show, in addition, that the copper sites in the two structures are distinctively different. The Met121Ala mutant still maintains the properties of an ordinary type 1 copper site while the Met121Ala-azide derivative has an absorption maximum at about 409 nm and the copper hyperfine coupling has increased to a value intermediate between those of type 2 copper and the wild-type azurin.
-===AZURIN MUTANT M121A-AZIDE===
+Mutant Met121Ala of Pseudomonas aeruginosa azurin and its azide derivative: crystal structures and spectral properties.,Tsai LC, Bonander N, Harata K, Karlsson G, Vanngard T, Langer V, Sjolin L Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):950-8. PMID:15299604<ref>PMID:15299604</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2tsb" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_15299604}}, adds the Publication Abstract to the page
+*[[Azurin 3D structures|Azurin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 15299604 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15299604}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-TSB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tsb 1tsb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TSB OCA].
+[[Category: Bonander, N]]
+[[Category: Harata, K]]
-==Reference==
+[[Category: Karlsson, B G]]
-Mutant Met121Ala of Pseudomonas aeruginosa azurin and its azide derivative: crystal structures and spectral properties., Tsai LC, Bonander N, Harata K, Karlsson G, Vanngard T, Langer V, Sjolin L, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):950-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299604 15299604]
+[[Category: Langer, V]]
-[[Category: Pseudomonas aeruginosa]]
+[[Category: Sjolin, L]]
-[[Category: Single protein]]
+[[Category: Tsai, L C]]
-[[Category: Bonander, N.]]
+[[Category: Vanngard, T]]
-[[Category: Harata, K.]]
-[[Category: Karlsson, B G.]]
-[[Category: Langer, V.]]
-[[Category: Sjolin, L.]]
-[[Category: Tsai, L C.]]
-[[Category: Vanngard, T.]]
 [[Category: Electron transport]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:50:21 2008''