Leucine transporter: Difference between revisions
No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| (3 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
<StructureSection load=' | <StructureSection load='' size='450' side='right' caption='Structure of leucine transporter complex with leucine, lipid membrane fragments and Na+ ions (purple) (PDB entry [[3usg]])' scene='56/564069/Cv/1' pspeed='8'> | ||
== Function == | == Function == | ||
'''Leucine transporter''' (LeuT) is a membrane protein which is comprised of 12 transmembrane helices forming a channel. LeuT clears the synapse after signal transduction. LeuT utilizes the electrochemical potential gradient of Na+ ions across the membrane to catalyze the uptake of leucine. <scene name='56/564069/Cv/5'>The LeuT is a homodimer</scene><ref>PMID:15980244</ref>. | '''Leucine transporter''' (LeuT) is a membrane protein which is comprised of 12 transmembrane helices forming a channel. LeuT clears the synapse after signal transduction. LeuT utilizes the electrochemical potential gradient of Na+ ions across the membrane to catalyze the uptake of leucine. <scene name='56/564069/Cv/5'>The LeuT is a homodimer</scene><ref>PMID:15980244</ref>. | ||
| Line 7: | Line 7: | ||
== Structural highlights == | == Structural highlights == | ||
*<scene name='56/564069/Cv/ | *<scene name='56/564069/Cv/6'>Leucine transporter complex with leucine, lipid membrane fragments and Na+ ions</scene>. | ||
*The <scene name='56/564069/Cv/ | *The <scene name='56/564069/Cv/7'>leucine binding pocket of LeuT contains 2 Na+ ions</scene><ref>PMID:22245965</ref>. | ||
*<scene name='56/564069/Cv/ | *<scene name='56/564069/Cv/8'>Leucine binding pocket</scene>. | ||
==3D structures of leucine transporter== | ==3D structures of leucine transporter== | ||
| Line 19: | Line 19: | ||
[[3mpn]], [[3mpq]] – AaLeuT (mutant) + Leu + Na + halide<br /> | [[3mpn]], [[3mpq]] – AaLeuT (mutant) + Leu + Na + halide<br /> | ||
[[3f3e]], [[3usg]], [[3usj]], [[3usi]], [[3usk]] – AaLeuT + Leu + Na <br /> | [[3f3e]], [[3usg]], [[3usj]], [[3usi]], [[3usk]] – AaLeuT + Leu + Na <br /> | ||
[[4fxz]], [[3gjc]] – AaLeuT (mutant) + Leu + Na <br /> | [[4fxz]], [[3gjc]], [[6nle]] – AaLeuT (mutant) + Leu + Na <br /> | ||
[[3f48]], [[3f4j]], [[3f3a]], [[3f3c]], [[3f3d]], [[3f4i]] – AaLeuT + amino acid + Na <br /> | [[3f48]], [[3f4j]], [[3f3a]], [[3f3c]], [[3f3d]], [[3f4i]], [[6xwm]] – AaLeuT + amino acid + Na <br /> | ||
[[3qs4]], [[3qs5]], [[3qs6]], [[4fy0]], [[3tu0]], [[3usl]], [[3uso]], [[3usm]] – AaLeuT (mutant) + amino acid + Na <br /> | [[3qs4]], [[3qs5]], [[3qs6]], [[4fy0]], [[3tu0]], [[3usl]], [[3uso]], [[3usm]], [[7lqj]], [[7lqk]], [[7lql]] – AaLeuT (mutant) + amino acid + Na <br /> | ||
[[3tt1]] – AaLeuT (mutant) + Na + antibody<br /> | [[3tt1]] – AaLeuT (mutant) + Na + antibody<br /> | ||
[[3tt3]] – AaLeuT (mutant) + antibody<br /> | [[3tt3]] – AaLeuT (mutant) + antibody<br /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | |||
Latest revision as of 11:26, 17 October 2021
<StructureSection load= size='450' side='right' caption='Structure of leucine transporter complex with leucine, lipid membrane fragments and Na+ ions (purple) (PDB entry 3usg)' scene='56/564069/Cv/1' pspeed='8'>
FunctionFunction
Leucine transporter (LeuT) is a membrane protein which is comprised of 12 transmembrane helices forming a channel. LeuT clears the synapse after signal transduction. LeuT utilizes the electrochemical potential gradient of Na+ ions across the membrane to catalyze the uptake of leucine. [1].
RelevanceRelevance
LeuT is the target of many antidepressant and recreational drugs.
Structural highlightsStructural highlights
- .
- The [2].
- .
3D structures of leucine transporter3D structures of leucine transporter
Updated on 17-October-2021
3gwu, 3gwv, 3gww, 2q6h, 2q72, 2qb4, 2qb4, 2qei – AaLeuT + Leu + Na + antidepressant – Aquifex aeolicus
2qju – AaLeuT (mutant) + Leu + Na + Cl + antidepressant
4hod, 4hmk, 3gjd, 3usp – AaLeuT + Leu + Na + halide
3mpn, 3mpq – AaLeuT (mutant) + Leu + Na + halide
3f3e, 3usg, 3usj, 3usi, 3usk – AaLeuT + Leu + Na
4fxz, 3gjc, 6nle – AaLeuT (mutant) + Leu + Na
3f48, 3f4j, 3f3a, 3f3c, 3f3d, 3f4i, 6xwm – AaLeuT + amino acid + Na
3qs4, 3qs5, 3qs6, 4fy0, 3tu0, 3usl, 3uso, 3usm, 7lqj, 7lqk, 7lql – AaLeuT (mutant) + amino acid + Na
3tt1 – AaLeuT (mutant) + Na + antibody
3tt3 – AaLeuT (mutant) + antibody
ReferencesReferences
- ↑ Tomi M, Mori M, Tachikawa M, Katayama K, Terasaki T, Hosoya K. L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier. Invest Ophthalmol Vis Sci. 2005 Jul;46(7):2522-30. PMID:15980244 doi:http://dx.doi.org/10.1167/iovs.04-1175
- ↑ Wang H, Elferich J, Gouaux E. Structures of LeuT in bicelles define conformation and substrate binding in a membrane-like context. Nat Struct Mol Biol. 2012 Jan 15;19(2):212-9. doi: 10.1038/nsmb.2215. PMID:22245965 doi:10.1038/nsmb.2215