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| __TOC__
| | ==Answers to Open-Ended Questions about Antibody== |
| ==Antibody Quiz== | | Here are answers to the [http://molviz.org/antibody/antibody-questions.pdf questions provided in a document] with the Antibody tutorial available through [http://molviz.org MolviZ.Org]. Suggestions and feedback to [[Special:Emailuser/Eric_Martz|Eric Martz]]. |
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| You will get immediate feedback when you click ''Submit'' (at the bottom of the quiz). The quiz below is offered to accompany the Antibody structure tutorial at [http://molviz.org MolviZ.Org]. For the open-ended questions provided with the tutorial, '''answers''' are [[#Answers to Open-Ended Questions|below]].
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| <!-- (i) = case Insensitive. _7 = width of slot (number of characters) for answer. -->
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| <quiz display=simple>
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| {How many "arms" does one antibody molecule have?
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| |type="{}"}
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| { 3|three (i)|three. (i) _7 }
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| {
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| |type="{}"}
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| How many '''Fab''' "arms" does one antibody molecule have?
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| { 2|two (i)|two. (i) _7 }
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| {
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| |type="{}"}
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| How many '''Fc''' "arms" does one antibody molecule have?
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| { 1|one (i)|one. (i) _7 }
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| {
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| |type="{}"}
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| How many CDRs are in one paratope?
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| { 6|six (i)|six. (i) _7 }
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| ||Three in the light chain variable domain plus three in the heavy chain variable domain.
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| {
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| |type="{}"}
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| What is the term for the smallest part of IgG1 that binds to antigen?
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| { Fv (i)|Fv. (i) _7 }
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| ||The variable fragment, containing only the variable domains: Fv.
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| {<hr>'''An IgG1 molecule has a molecular weight of 150 kilo Daltons.'''}
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| {
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| |type="{}"}
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| What is the approximate molecular weight of one immunoglobulin domain in kD?
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| { 10-13 _7 } kD.
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| ||Twelve Ig domains in one IgG1 molecule. 150/12 = 12.5 kD.
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| {
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| |type="{}"}
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| What is the approximate molecular weight of a single Fc in kD?
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| { 48-55 _7 } kD.
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| ||Fc is one of three roughly equal-sized arms, each containing 8 Ig domains. 150/3 = 50 kD.
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| {<hr>
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| '''Questions below may have <font color="red">more than one</font> correct answer.'''}
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| {Antibody
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| |type="[]"}
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| + Binds to antigen.
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| + When bound to a microbe, recruits macrophages and other leukocytes to kill the microbes.
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| + Defends us against infections.
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| - Carries oxygen to tissues.
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| - Is a hormone.
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| - Strengthens bones.
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| {Antibody molecules consist of
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| |type="[]"}
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| + Protein.
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| - Lipid.
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| - Salt.
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| + Sugars.
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| + Carbohydrate.
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| + Amino acids.
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| - Fatty acids.
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| + Immunoglobulin domains.
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| {Antibody is manufactured by
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| |type="[]"}
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| - T lymphocytes.
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| - The liver.
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| + B lymphocytes.
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| - Antigen.
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| - G lymphocytes.
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| - Macrophages.
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| {Variable immunoglobulin domains
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| |type="[]"}
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| + Are in Fab.
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| - Are in Fc.
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| + Are in all antibodies.
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| - Have no disulfide bonds.
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| - Do not contain CDRs.
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| - Make up the epitope.
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| + Make up the paratope.
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| - Have the same amino acid sequence in all antibodies of a given subclass.
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| {A single immunoglobulin domain contains
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| |type="[]"}
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| - Three beta sheets.
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| + Two beta sheets.
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| - One beta sheet.
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| - Two alpha helices.
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| - One alpha helix.
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| + One disulfide bond.
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| - Two disulfide bonds.
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| - A hydrophilic core.
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| + A hydrophobic core.
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| {Which CDRs contribute the most to antigen specificity?
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| |type="[]"}
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| - Light chain CDRs.
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| - Heavy chain CDRs.
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| - CDR1s.
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| - CDR2s.
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| + CDR3s.
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| - CDR4s.
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| </quiz>
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| Suggestions to emartz at microbio dot umass dot edu.
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| Educators are invited to copy this quiz into a Proteopedia page of your own, where you
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| could delete some questions and add some of your own. (Click the tab ''edit this page''
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| at the top, then block and copy everything in the box. Paste that into the wikitext box
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| of your own new page, and save the page. See [[Proteopedia:How to Make a Page]].)
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| ==Answers to Open-Ended Questions==
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| Here are answers to the questions provided in a document with the Antibody tutorial.
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| <ol><li> | | <ol><li> |
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| <!--15-->The analogy is to a single immunoglobulin domain. The two beta sheets are the "bread"; the butter is the hydrophobic core; and the toothpick represents the disulfide bond. | | <!--15-->The analogy is to a single immunoglobulin domain. The two beta sheets are the "bread"; the butter is the hydrophobic core; and the toothpick represents the disulfide bond. |
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| | | </li></ol> |
| [[Category:Pages with quizzes]]
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