Human lactoferrin: Difference between revisions
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Undo revision 3437870 by Karsten Theis (Talk) |
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The structure of LF<sub>N</sub> undergoes a dramatic conformational change upon iron binding. Upon iron binding, the two domains of LF<sub>N</sub> undergo a rigid 54.1º rotation about a <scene name='Sandbox_Reserved_302/Hinge/1'>screw axis</scene> that passes through Thr90 and Pro251.<ref name="gerstein">PMID:8230220</ref> | The structure of LF<sub>N</sub> undergoes a dramatic conformational change upon iron binding. Upon iron binding, the two domains of LF<sub>N</sub> undergo a rigid 54.1º rotation about a <scene name='Sandbox_Reserved_302/Hinge/1'>screw axis</scene> that passes through Thr90 and Pro251.<ref name="gerstein">PMID:8230220</ref> | ||
To visualize the <jmol> | |||
<jmolLink> | |||
<script>load files "=1CB6" "=1LCF";model 0;cartoon only;domain2 = {(92-249 or 1092-1249)};select domain2 and _C;color green; | |||
structures = [{1.1}, {2.1}]; | |||
domains = [[{not domain2},{not domain2}, {alpha and not domain2 and not altloc="B"}], [{domain2}, {(91,1091,250,1250) and *.CA}, {alpha and domain2 and not altloc="B"}],];moveto 1.0 { 880 471 63 56.8} 132.25 0.0 0.0 {2.646000000000001 15.972 -7.2715} 63.954979842981245 {0 0 0} 0 0 0 3.0 0.0 0.0; | |||
script "https://proteopedia.org/wiki/images/a/a2/Storymorph.spt";superimpose(structures,domains,1);model 1;delay 0.5;model 2;delay 0.5;model 1; | |||
</script> | |||
<text>domain motion</text> | |||
</jmolLink> | |||
</jmol>, we first load the two structures and superimpose them. You can choose a viewing orientation before pressing the morph button, which will visualize the conformational change from structure 1CB6 to 1LCF and back again<ref>The [[Jmol/Storymorph|Storymorph Jmol scripts]] creates the interpolated coordinates of the morph on the fly.</ref> | |||
. | |||
<jmol> | |||
<jmolButton> | |||
<script>morph_palindrome = 1; | |||
morph(15,structures,domains)</script> | |||
<text>Morph</text> | |||
</jmolButton> | |||
</jmol> <jmol> | |||
<jmolButton> | |||
<script>select protein;spacefill only;</script> | |||
<text>spacefill</text> | |||
</jmolButton> | |||
</jmol> | |||
=Function & Application= | =Function & Application= | ||
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<references /> | <references /> | ||
Page originally authored by | Page originally authored by [http://proteopedia.org/wiki/index.php/User:Christian_Axen Christian Axen] | ||