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{{Theoretical_model}} | |||
==STRUCTURE OF THE TRANSLATING 80S RIBOSOME FROM YEAST, OBTAINED BY DOCKING ATOMIC MODELS FOR RNA AND PROTEIN COMPONENTS INTO A 15A CRYO-EM MAP. THIS FILE 1K5Y CONTAINS THE 60S RIBOSOMAL SUBUNIT. THE FILE 1K5X CONTAINS THE 40S RIBOSOMAL SUBUNIT, THE P-SITE BOUND TRNA AND THE MRNA CODON.== | |||
<StructureSection load='1k5y' size='340' side='right'caption='[[1k5y]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K5Y FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k5y FirstGlance], [https://www.ebi.ac.uk/pdbsum/1k5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k5y ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A cryo-EM reconstruction of the translating yeast 80S ribosome was analyzed. Computationally separated rRNA and protein densities were used for docking of appropriately modified rRNA models and homology models of yeast ribosomal proteins. The core of the ribosome shows a remarkable degree of conservation. However, some significant differences in functionally important regions and dramatic changes in the periphery due to expansion segments and additional ribosomal proteins are evident. As in the bacterial ribosome, bridges between the subunits are mainly formed by RNA contacts. Four new bridges are present at the periphery. The position of the P site tRNA coincides precisely with its prokaryotic counterpart, with mainly rRNA contributing to its molecular environment. This analysis presents an exhaustive inventory of an eukaryotic ribosome at the molecular level. | |||
Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.,Spahn CM, Beckmann R, Eswar N, Penczek PA, Sali A, Blobel G, Frank J Cell. 2001 Nov 2;107(3):373-86. PMID:11701127<ref>PMID:11701127</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1k5y" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Theoretical Model]] | |||
[[Category: Large Structures]] | |||
[[Category: Beckmann, R]] | |||
[[Category: Blobel, G]] | |||
[[Category: Eswar, N]] | |||
[[Category: Frank, J]] | |||
[[Category: Penczek, P]] | |||
[[Category: Sali, A]] | |||
[[Category: Spahn, C M.T]] | |||
Latest revision as of 09:49, 11 August 2021
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STRUCTURE OF THE TRANSLATING 80S RIBOSOME FROM YEAST, OBTAINED BY DOCKING ATOMIC MODELS FOR RNA AND PROTEIN COMPONENTS INTO A 15A CRYO-EM MAP. THIS FILE 1K5Y CONTAINS THE 60S RIBOSOMAL SUBUNIT. THE FILE 1K5X CONTAINS THE 40S RIBOSOMAL SUBUNIT, THE P-SITE BOUND TRNA AND THE MRNA CODON.STRUCTURE OF THE TRANSLATING 80S RIBOSOME FROM YEAST, OBTAINED BY DOCKING ATOMIC MODELS FOR RNA AND PROTEIN COMPONENTS INTO A 15A CRYO-EM MAP. THIS FILE 1K5Y CONTAINS THE 60S RIBOSOMAL SUBUNIT. THE FILE 1K5X CONTAINS THE 40S RIBOSOMAL SUBUNIT, THE P-SITE BOUND TRNA AND THE MRNA CODON.
Structural highlights
Publication Abstract from PubMedA cryo-EM reconstruction of the translating yeast 80S ribosome was analyzed. Computationally separated rRNA and protein densities were used for docking of appropriately modified rRNA models and homology models of yeast ribosomal proteins. The core of the ribosome shows a remarkable degree of conservation. However, some significant differences in functionally important regions and dramatic changes in the periphery due to expansion segments and additional ribosomal proteins are evident. As in the bacterial ribosome, bridges between the subunits are mainly formed by RNA contacts. Four new bridges are present at the periphery. The position of the P site tRNA coincides precisely with its prokaryotic counterpart, with mainly rRNA contributing to its molecular environment. This analysis presents an exhaustive inventory of an eukaryotic ribosome at the molecular level. Structure of the 80S ribosome from Saccharomyces cerevisiae--tRNA-ribosome and subunit-subunit interactions.,Spahn CM, Beckmann R, Eswar N, Penczek PA, Sali A, Blobel G, Frank J Cell. 2001 Nov 2;107(3):373-86. PMID:11701127[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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