FIS protein: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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+<StructureSection load='3jra' size='350' side='right' scene='54/542232/Cv/1' caption='FIS protein complex with DNA  [[3jra]]'>
-[[Image:3jra.png|left|200px|thumb|Crystal structure of FIS protein complex with DNA [[3jra]]]]
+'''FIS protein (Factor for Inversion Stimulation)''' or '''DNA-binding protein Fis''' is a regulatory protein in ''E. coli'' that activates ribosomal RNA transcription by binding to RNA promoter and enhances transcription by 5 to 10-fold.  FIS binds to a sequence which stimulates hin-mediated DNA inversion and causes DNA bending.  FIS affects the bacterial chromosome structure and the initiation of DNA replication.   FIS exhibits autoregulation.  It is abundant during ''E. coli'' exponential growth in rich medium and scarce during the stationary phase<ref>PMID:11278071</ref>.
-{{STRUCTURE_3jra|  PDB=3jra  | SIZE=400| SCENE= |right|CAPTION=FIS protein complex with DNA  [[3jra]] }}
+*<scene name='54/542232/Cv/2'>FIS protein complex with DNA</scene> ([[3jra]]).
+*<scene name='54/542232/Cv/5'>Protein/DNA interactions</scene>.
+*<scene name='54/542232/Cv/4'>Protein/DNA interactions (details, chain A)</scene>.
+*<scene name='54/542232/Cv/3'>Protein/DNA interactions (details, chain B)</scene>.
+</StructureSection>
-'''FIS protein (Factor for Inversion Stimulation)''' is active in gene regulation like recombination, transcriptional activation, DNA replication and self repression.  Cellular concentration of FIS increases 500-fold prior to cell division.  FIS binds to RNA activation sites and enhances transcription.
 == 3D Structures of FIS protein ==
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 [[1fia]], [[3fis]], [[4fis]], [[1ety]] – EcFIS - ''Escherichia coli'' <br />
 [[1f36]], [[1etk]], [[1eto]], [[1etq]], [[1etv]], [[1etw]], [[1etx]]  – EcFIS (mutant) <br />
-[[1fip]] – EcFIS + peptide <br />
+[[1fip]] – EcFIS (mutant) + peptide <br />
-[[3iv5]], [[3jr9]], [[3jra]], [[3jrb]], [[3jrc]] [[3jrd]], [[3jre]], [[3jrf]], [[3jrg]], [[3jrh]], [[3jri]] – EcFIS + DNA <br />
+[[3iv5]], [[3jr9]], [[3jra]], [[3jrb]], [[3jrc]], [[3jrd]], [[3jre]], [[3jrf]], [[3jrg]], [[3jrh]], [[3jri]], [[4ihv]], [[4ihw]], [[4ihx]], [[4ihy]], [[5ds9]], [[5dtd]], [[5e3l]], [[5e3m]], [[5e3n]], [[5e3o]], [[6p0s]] – EcFIS + DNA <br />
+[[6p0t]], [[6p0u]]  – EcFIS + DNA + exicionase<br />
+== References ==
+<references/>
+[[Category:Topic Page]]