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Deoxyuridine 5'-triphosphate nucleotidohydrolase: Difference between revisions

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<StructureSection load='1w2y' size='450' side='right' caption='dUTPase complex with dUTP analog and Mg+2 ions (green) (PDB entry [[1w2y]])' scene='48/488500/Cv/1'>
<StructureSection load='1w2y' size='350' side='right' caption='dUTPase complex with dUTP analog and Mg+2 ions (green) (PDB entry [[1w2y]])' scene='48/488500/Cv/1'>


== Function ==
== Function ==


'''Deoxyuridine 5’-triphosphate nucleotidohydrolase''' (DUTP) catalyzes the conversion of dUTP to dUMP and pyrophosphate (PPi).  DUTP plays a key role in keeping significant amounts of dUTP from the DNA synthesis pathway.  
'''Deoxyuridine 5’-triphosphate nucleotidohydrolase''' or '''Deoxyuridine triphosphatase''' or '''dUTP pyrophosphatase''' (DUTP) catalyzes the conversion of dUTP to dUMP and pyrophosphate (PPi).  DUTP plays a key role in keeping significant amounts of dUTP from the DNA synthesis pathway.  
Prokaryotic DUTP contains metal ion. <ref>PMID:18837522</ref>
Prokaryotic DUTP contains metal ion. <ref>PMID:18837522</ref>


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== Structural highlights ==
== Structural highlights ==


The <scene name='48/488500/Cv/3'>active site</scene> contains Mg<sup>+2</sup> ions which are essensial for DUTP activity. The <scene name='48/488500/Cv/4'>Mg+2 ions are hexacoordinated to acidic residues and water molecules</scene>.<ref>PMID:15364583</ref>
The <scene name='48/488500/Cv/5'>active site</scene> contains Mg<sup>+2</sup> ions which are essensial for DUTP activity. The <scene name='48/488500/Cv/6'>Mg+2 ions are hexacoordinated to acidic residues and water molecules</scene>.<ref>PMID:15364583</ref> Water molecules are labeled Wa.
</StructureSection>
 
==3D structures of dUTPase==
==3D structures of dUTPase==
[[dUTPase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*DUTP
 
**[[1dup]], [[1eu5]], [[1euw]] – EcDUTP – ''Escherichia coli''<br />
**[[1dun]] – EvDUTP – Equine infectious anemia virus<br />
**[[1mq7]] – MtDUTP – ''Mycobacterium tuberculosis''<br />
**[[4gk6]] - DUTP – ''Mycobacterium abscessus''<br />
**[[1ogl]] – TcDUTP – ''Trypanosoma cruzi''<br />
**[[2d4l]] - MPmvDUTP residues 83-234 – Mason_Pfizer monkey virus<br />
**[[2d4m]] - MPmvDUTP residues 83-234 (mutant) <br />
**[[2okb]], [[2okd]] - VvDUTP – Vaccinia virus<br />
**[[3hhq]] - yDUTP – yeast<br />
**[[3lqw]] - DUTP – ''Entamoeba histolytica''<br />
**[[3mbq]], [[3mdx]] - DUTP – ''Brucella melitensis''<br />
**[[2xx6]] - BsDUTP – ''Bacillus subtilis''<br />
**[[3tqz]] - DUTP – ''Coxiella burnetii''<br />
**[[1q5u]] - hDUTP – human<br />
**[[4lhr]] - DUTP – ''Burkholderia thailandensis''<br />
 
*DUTP binary complexes
 
**[[1dud]] – EcDUTP + dUDP<br />
**[[2hrm]] - EcDUTP + dUTP derivative<br />
**[[1seh]] - EcDUTP + dUMP<br />
**[[3f4f]] - yDUTP + dUMP<br />
**[[1dut]] – DUTP + Mg – Feline immunodeficiency virus<br />
**[[1ogk]] – TcDUTP + dUTP<br />
**[[1smc]] - MtDUTP + dUTP<br />
**[[4gcy]] - MtDUTP (mutant) + dUTP<br />
**[[1vyq]], [[3t60]], [[3t64]], [[3t6y]], [[3t70]] - PfDUTP + uridine derivative inhibitor – ''Plasmodium falciparum''<br />
**[[2y8c]] - PfDUTP + trityl ligand<br />
**[[2bsy]], [[2we0]] - EbvDUTP + dUMP – Epstein-Barr virus<br />
**[[2we1]], [[2we2]] - EbvDUTP (mutant) + dUMP<br />
**[[2ol1]] - VvDUTP + dUMP<br />
**[[2y1t]] - BsDUTP + dUDP<br />
**[[4ao5]] - BsDUTP + dUMP<br />
**[[2yb0]] - LmDUTP + dU – ''Leishmania major''<br />
**[[3tq5]], [[3trl]], [[3trn]], [[3ts6]], [[3tsl]], [[3tta]] - MPmvDUTP catalytic domain (mutant) + dUMP<br />
**[[2p9o]], [[2pc5]] – DUTP + Mg – ''Arabidopsis thaliana''<br />
**[[4mz5]], [[4mz6]] – hDUTP + importin subunit α-1<br />
 
*DUTP ternary complexes


**[[1duc]] – EvDUPT + Sr + dUTP<br />
**[[1six]], [[1sjn]], [[2py4]] – MtDUTP + Mg + dUTP derivative<br />
**[[3h6d]], [[3hza]], [[3i93]] - MtDUTP (mutant) + Mg + dUTP derivative<br />
**[[3loj]] - MtDUTP (mutant) + Mg + Mn + dUTP derivative<br />
**[[1rn8]] - EcDUTP + Mg + dUTP derivative<br />
**[[1rnj]] - EcDUTP (mutant) + Mg + dUTP derivative<br />
**[[1syl]] - EcDUTP (mutant) + Mg + dUTP<br />
**[[2hr6]] - EcDUTP + Mn + dUDP<br />
**[[1slh]] – MtDUTP + Mg + dUDP<br />
**[[1sm8]] – MtDUTP + Cr + dUTP<br />
**[[1snf]] - MtDUTP + Mg + dUMP<br />
**[[1w2y]], [[2cic]] - DUTP + Mg + dUTP derivative – ''Campylobacter jejuni''<br />
**[[2bt1]] - EbvDUTP + Mg + dUTP derivative<br />
**[[2we3]] - EbvDUTP residues 1-256 + Mg + dUTP<br />
**[[2d4n]] - MPmvDUTP residues 83-234 (mutant) + Mg + dUTP derivative<br />
**[[3tp1]] - MPmvDUTP catalytic domain + Mg + dUTP derivative<br />
**[[3tpw]], [[3tpn]], [[3tps]] - MPmvDUTP catalytic domain (mutant) + Mg + dUTP derivative<br />
**[[3tpy]], [[3tq3]], [[3tq4]] - MPmvDUTP catalytic domain + Mg + dUMP + dUTP derivative<br />
**[[2cje]] - LmDUTP + Mg + dUTP derivative<br />
**[[2yay]] - LmDUTP + Ca + dUTP derivative<br />
**[[2yaz]] - LmDUTP + Mg + dUMP<br />
**[[2oke]] - VvDUTP + Mg + dUTP derivative<br />
**[[2ol0]] - VvDUTP + Mg + dUDP<br />
**[[3c3i]] - PbcvDUTP (mutant) + Mg + dUDP – Paramecium bursaria chlorella virus<br />
**[[3c2t]], [[3ca9]] - PbcvDUTP + Mg + dUDP<br />
**[[2hqu]] - hDUTP + Mg + dUTP derivative <br />
**[[1q5h]] - hDUTP + Mg + dUDP<br />
**[[3ehw]] - hDUTP + Mg + dUTP<br />
**[[3ara]], [[3arn]] - hDUTP + Mg + uracil derivative<br />
**[[3p48]] - yDUTP + Mg + dUTP derivative<br />
**[[2xy3]] - BsDUTP + Mg + dUTP derivative<br />
**[[4aoo]], [[4apz]], [[4aoz]] - BsDUTP + Mg + PPi + uridine<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky
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