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| <!-- | | <StructureSection load='1w2y' size='350' side='right' caption='dUTPase complex with dUTP analog and Mg+2 ions (green) (PDB entry [[1w2y]])' scene='48/488500/Cv/1'> |
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| '''Deoxyuridine 5’-triphosphate nucleotidohydrolase''' (DUTP) catalyzes the conversion of dUTP to dUMP and pyrophosphate (PPi). DUTP plays a key role in keeping significant amounts of dUTP from the DNA synthesis pathway. DUTP inhibitors are being tested as possible anti-bacterial agents targeting diseases like malaria, leishmaniasis, tuberculosis and trypanosomiasis. Prokaryotic DUTP contains metal ion. | |
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| ==3D structures of dTPase== | | == Function == |
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| [[1dup]], [[1eu5]], [[1euw – EcDUTP – ''Escherichia coli''<br />
| | '''Deoxyuridine 5’-triphosphate nucleotidohydrolase''' or '''Deoxyuridine triphosphatase''' or '''dUTP pyrophosphatase''' (DUTP) catalyzes the conversion of dUTP to dUMP and pyrophosphate (PPi). DUTP plays a key role in keeping significant amounts of dUTP from the DNA synthesis pathway. |
| [[1dun]] – EvDUTP – Equine infectious anemia virus<br />
| | Prokaryotic DUTP contains metal ion. <ref>PMID:18837522</ref> |
| [[1mq7]] – MtDUTP – ''Mycobacterium tuberculosis''<br />
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| [[1ogl]] – TcDUTP – ''Trypanosoma cruzi''<br />
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| [[2d4l]] - MPmvDUTP residues 83-234 – Mason_Pfizer monkey virus<br />
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| [[2d4m]] - MPmvDUTP residues 83-234 (mutant) <br />
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| [[2okb]], [[2okd]] - VvDUTP – Vaccinia virus<br />
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| [[3hhq]] - yDUTP – yeast<br />
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| [[3lqw]] - DUTP – ''Entamoeba histolytica''<br />
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| [[3mbq]], [[3mdx]] - DUTP – ''Brucella melitensis''<br />
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| [[2xx6]] - BsDUTP – ''Bacillus subtilis''<br />
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| [[3tqz]] - DUTP – ''Coxiella burnetii''
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| '''DUTP binary complexes'''
| | == Relevance == |
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| [[1dud]] – EcDUTP + dUDP<br />
| | DUTP inhibitors are being tested as possible anti-bacterial agents targeting diseases like malaria, leishmaniasis, tuberculosis and trypanosomiasis. |
| [[2hrm]] - EcDUTP + dUTP derivative<br />
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| [[1seh]] - EcDUTP + dUMP<br />
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| [[3f4f]] - yDUTP + dUMP<br />
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| [[1dut]] – DUTP + Mg – Feline immunodeficiency virus<br />
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| [[1ogk]] – TcDUTP + dUTP<br />
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| [[1smc]] - MtDUTP + dUTP<br />
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| [[1vyq]] - PfDUTP + inhibitor – ''Plasmodium falciparum''<br />
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| [[2y8c]] - PfDUTP + trityl ligand<br />
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| [[2bsy]], [[2we0]] - EbvDUTP + dUMP – Epstein-Barr virus<br />
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| [[2we1]], [[2we2]] - EbvDUTP (mutant) + dUMP<br />
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| [[2ol1]] - VvDUTP + dUMP<br />
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| [[2y1t]] - BsDUTP + dUDP<br />
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| [[2yb0]] - LmDUTP + dU – ''Leishmania major''<br />
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| [[3tq5]], [[3trl]], [[3trn]], [[3ts6]], [[3tsl]], [[3tta]] - MPmvDUTP catalytic domain (mutant) + dUMP<br />
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| '''DUTP ternary complexes'''
| | == Structural highlights == |
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| [[1duc]] – EvDUPT + Sr + dUTP<br />
| | The <scene name='48/488500/Cv/5'>active site</scene> contains Mg<sup>+2</sup> ions which are essensial for DUTP activity. The <scene name='48/488500/Cv/6'>Mg+2 ions are hexacoordinated to acidic residues and water molecules</scene>.<ref>PMID:15364583</ref> Water molecules are labeled Wa. |
| [[1six]], [[1sjn]], [[2py4]] – MtDUTP + Mg + dUTP derivative<br />
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| [[3h6d]], [[3hza]], [[3i93]] - MtDUTP (mutant) + Mg + dUTP derivative<br />
| | ==3D structures of dUTPase== |
| [[3loj]] - MtDUTP (mutant) + Mg + Mn + dUTP derivative<br />
| | [[dUTPase 3D structures]] |
| [[1rn8]] - EcDUTP + Mg + dUTP derivative<br />
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| [[1rnj]] - EcDUTP (mutant) + Mg + dUTP derivative<br />
| | </StructureSection> |
| [[1syl]] - EcDUTP (mutant) + Mg + dUTP<br />
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| [[2hr6]] - EcDUTP + Mn + dUDP<br />
| | == References == |
| [[1slh]] – MtDUTP + Mg + dUTP<br />
| | <references/> |
| [[1sm8]] – MtDUTP + Cr + dUTP<br />
| | [[Category:Topic Page]] |
| [[1snf]] - MtDUTP + Mg + dUMP<br />
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| [[1w2y]], [[2cic]] - DUTP + Mg + dUTP derivative – ''Campylobacter jejuni''<br />
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| [[2bt1]] - EbvDUTP + Mg + dUTP derivative<br />
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| [[2we3]] - EbvDUTP residues 1-256 + Mg + dUTP<br />
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| [[2d4n]] - MPmvDUTP residues 83-234 (mutant) + Mg + dUTP derivative<br />
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| [[3tp1]] - MPmvDUTP catalytic domain + Mg + dUTP derivative<br />
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| [[3tpw]], [[3tpn]], [[3tps]] - MPmvDUTP catalytic domain (mutant) + Mg + dUTP derivative<br />
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| [[3tpy]], [[3tq3]], [[3tq4]] - MPmvDUTP catalytic domain + Mg + dUMP + dUTP derivative<br />
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| [[2cje]] - LmDUTP + Mg + dUTP derivative<br />
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| [[2yay]] - LmDUTP + Ca + dUTP derivative<br />
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| [[2yaz]] - LmDUTP + Mg + dUMP<br />
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| [[2oke]] - VvDUTP + Mg + dUTP derivative<br />
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| [[2ol0]] - VvDUTP + Mg + dUDP<br /> | |
| [[3c3i]] - PbcvDUTP (mutant) + Mg + dUDP – Paramecium bursaria chlorella virus<br />
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| [[3c2t]], [[3ca9]] - PbcvDUTP + Mg + dUDP<br />
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| [[2hqu]] - hDUTP + Mg + dUTP derivative – human<br />
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| [[3ara]], [[3arn]] - hDUTP + Mg + uracil derivative<br />
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| [[3p48]] - yDUTP + Mg + dUTP derivative<br />
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| [[2xy3]] - BsDUTP + Mg + dUTP derivative<br /> | |