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| <StructureSection load='2qcd' size='340' side='right' caption='Human uridine 5-monophosphate synthase OPD subunit dimer complex with UMP [[2qcd]]' scene='' > | | <StructureSection load='' size='350' side='right' caption='Human uridine 5-monophosphate synthase OPD subunit dimer complex with UMP [[2qcd]]' scene='52/526172/Cv/1' pspeed='8'> |
| == Function == | | == Function == |
| '''Uridine 5’-monophosphate synthase''' (UMPS) is a bifunctional enzyme catalyzes the formation of uridine monophosphate (UMP)<ref>PMID:8631878</ref>. UMPS N-terminal domain is an '''orotate phosphoribosyl transferase''' (OPRT) subunit which catalyzes the addition of ribose-phosphate to orotate forming orotidine 5’-monophosphate (OMP). The C-terminal subunit is '''orotidine 5’-phosphate decarboxylase''' (OPD) which decarboxylates OMP to form UMP. A potent inhibitor of OPD is BMP – a barbituric acid derivative. | | '''Uridine 5’-monophosphate synthase''' (UMPS) is a bifunctional enzyme which catalyzes the formation of uridine monophosphate (UMP)<ref>PMID:8631878</ref>. UMPS '''N-terminal domain''' is an '''orotate phosphoribosyltransferase''' (OPRT) subunit which catalyzes the addition of ribose-phosphate to orotate forming orotidine 5’-monophosphate (OMP). The '''C-terminal subunit''' is '''orotidine 5’-phosphate decarboxylase''' (OPD) or '''OMP decarboxylase''' which decarboxylates OMP to form UMP. Potent inhibitors of OPD are BMP – a barbituric acid derivative and xanthosine-5'-monophosphate (XMP). |
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| == Disease == | | == Disease == |
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| == Structural highlights == | | == Structural highlights == |
| UMPS contains UMP in its nucleotide-binding pocket<ref>PMID:18184586</ref>. | | <scene name='52/526172/Cv/8'>UMPS contains UMP</scene> in its <scene name='52/526172/Cv/9'>nucleotide-binding pocket</scene><ref>PMID:18184586</ref> (the surface of chain B doesn't shown). Water molecules are shown as red spheres. |
| | ==3D structures of uridine 5'-monophosphate synthase== |
| | [[Uridine 5'-monophosphate synthase 3D structures]] |
| </StructureSection> | | </StructureSection> |
| ==3D structures of uridine 5'-monophosphate synthase==
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Uridine 5’-monophosphate synthase OPD subunit
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| **[[3gdk]] - yOPD – yeast<br />
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| **[[1dqw]], [[3gdr]], [[3gdm]] – yOPD (mutant) <br />
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| **[[2eaw]], [[2jgy]], [[2p1f]], [[2qcc]], [[2qce]] - hOPD – human<br />
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| **[[4n2y]] – AfOPD – ''Archaeoglobus fulgidus''<br />
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| **[[4lui]] – MjOPD – ''Methanocaldococcus jannaschii''<br />
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| **[[4dbd]] – SsOPD – ''Sulfolobus solftaricus'' <br />
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| **[[4df0]] – TnOPD – ''Thermoproteus neutrophilus'' <br />
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| **[[3ve9]] – MsOPD – ''Metallosphaera sedula'' <br />
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| *Uridine 5’-monophosphate synthase OPD subunit complex
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| **[[2v30]], [[2qcd]] - hOPD + UMP<br />
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| **[[3ewy]] - hOPD (mutant) + UMP<br />
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| **[[3gdl]] - yOPD + UMP derivative<br />
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| **[[1dqx]], [[3gdt]] - yOPD (mutant) + UMP derivative<br />
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| **[[2qcg]], [[2qch]], [[3bgg]], [[3bgj]], [[3bk0]], [[3g3d]], [[3g3m]], [[3ewz]], [[3ex2]], [[3ex3]], [[3ex4]], [[3dbp]], [[3l0k]], [[3l0n]], [[3mo7]], [[3mw7]] - hOPD + UMP derivative<br />
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| **[[3bvj]] - hOPD + xanthosine 5’-monophosphate<br />
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| **[[2qcf]], [[2qcm]], [[2qcn]], [[3ewu]], [[3ewx]], [[3ex6]] - hOPD (mutant) + UMP derivative<br />
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| **[[3mi2]], [[4hib]], [[4hkp]] - hOPD + inhibitor<br />
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| **[[3ex1]] - hOPD + UMP derivative + UMP<br />
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| **[[2qcl]] - yOPD (mutant) + OMP <br />
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| **[[4lw7]] – MtOPD + BMP – ''Methanothermobacter thermautotrophicus''<br />
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| **[[4lc6]], [[4lc8]], [[4fx8]], [[4gc4]], [[4fx6]], [[4fxr]], [[3v1p]] – MtOPD (mutant) + BMP <br />
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| **[[4o8r]], [[4o11]], [[4nx5]], [[4nt0]], [[4nuw]], [[3wjw]], [[3wjx]], [[3wjy]], [[3wjz]], [[3w07]] - MtOPD + UMP derivative<br />
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| **[[3wk0]], [[3wk1]], [[3wk2]], [[3wk3]] - MtOPD + OMP derivative<br />
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| **[[4muz]] – AfOPD + BMP <br />
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| **[[4luj]] – MjOPD + BMP <br />
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| **[[4df1]] – TnOPD + BMP <br />
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| **[[4dbe]] – SsOPD + BMP <br />
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| **[[3ve7]] – MsOPD + BMP <br />
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| *Uridine 5’-monophosphate synthase OPRT subunit complex
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| **[[2wns]] - hOPRT + OMP<br />
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| **[[4wml]] – yOPRT + phosphoribosyl pyrophosphate<br />
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| **[[1lh0]], [[1opr]] – StOPRT + MgPRPP + orotate – ''Salmonella typhimurium''<br />
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| }}
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| ==References== | | ==References== |
| <references /> | | <references /> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |