Uridine 5'-monophosphate synthase: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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+<StructureSection load='' size='350' side='right' caption='Human uridine 5-monophosphate synthase OPD subunit dimer complex with UMP [[2qcd]]' scene='52/526172/Cv/1' pspeed='8'>
-'''Uridine 5’-monophosphate synthase''' (UMPS) is a bifunctional enzyme catalyzes the formation of uridine monophosphate (UMP).  UMPS N-terminal domain is an orotate phosphoribosyl transferase (OPRT) subunit which catalyzes the addition of ribose-phosphate to orotate forming orotidine 5’-monophosphate (OMP).  The C-terminal subunit is orotidine 5’-phosphate decarboxylase (OPD) which decarboxylates OMP to form UMP.
+== Function ==
+'''Uridine 5’-monophosphate synthase''' (UMPS) is a bifunctional enzyme which catalyzes the formation of uridine monophosphate (UMP)<ref>PMID:8631878</ref>.  UMPS '''N-terminal domain''' is an '''orotate phosphoribosyltransferase''' (OPRT) subunit which catalyzes the addition of ribose-phosphate to orotate forming orotidine 5’-monophosphate (OMP).  The '''C-terminal subunit''' is '''orotidine 5’-phosphate decarboxylase''' (OPD) or '''OMP decarboxylase''' which decarboxylates OMP to form UMP. Potent inhibitors of OPD are BMP – a barbituric acid derivative and xanthosine-5'-monophosphate (XMP).
-===3D structures of uridine 5'-monophosphate synthase===
+== Disease ==
+Defects in UMPS result in the hereditary rare metabolic disease orotic aciduria<ref>PMID:25757096</ref>.  UMPS deficiency in Holstein cattle results in an autosomal disorder which causes early embryonic death of offspring<ref>PMID:8486364</ref>.
-==Uridine 5’-monophosphate synthase OPD subunit==
+== Structural highlights ==
+<scene name='52/526172/Cv/8'>UMPS contains UMP</scene> in its <scene name='52/526172/Cv/9'>nucleotide-binding pocket</scene><ref>PMID:18184586</ref> (the surface of chain B doesn't shown). Water molecules are shown as red spheres.
+==3D structures of uridine 5'-monophosphate synthase==
+[[Uridine 5'-monophosphate synthase 3D structures]]
+</StructureSection>
-[[3gdk]] - yOPD]] – yeast<br />
+==References==
-[[1dqw]], [[3gdr]], [[3gdm – yOPD (mutant) <br />
+<references />
-[[2eaw]], [[2jgy]], [[2p1f]], [[2qcc]], [[2qce]] - hOPD – human
+[[Category:Topic Page]]
-==Uridine 5’-monophosphate synthase OPD subunit complex==
-[[2v30]], [[2qcd]] - hOPD + UMP<br />
-[[3ewy]] - hOPD (mutant) + UMP<br />
-[[3gdl]] - yOPD + UMP derivative<br />
-[[1dqx]], [[3gdt]] - yOPD (mutant) + UMP derivative<br />
-[[2qcg]], [[2qch]], [[3bgg]], [[3bgj]], [[3bk0]], [[3g3d]], [[3g3m]], [[3ewz]], [[3ex2]], [[3ex3]], [[3ex4]], [[3dbp]], [[3l0k]], [[3l0n]], [[3mo7]], [[3mw7]] - hOPD + UMP derivative<br />
-[[3bvj]] - hOPD + xanthosine 5’-monophosphate<br />
-[[2qcf]], [[2qcm]], [[2qcn]], [[3ewu]], [[3ewx]], [[3ex6]] - hOPD (mutant) + UMP derivative<br />
-[[3mi2]], [[4hib]], [[4hkp]] - hOPD + inhibitor<br />
-[[3ex1]] - hOPD + UMP derivative + UMP<br />
-[[2qcl]] - yOPD (mutant) + OMP
-==Uridine 5’-monophosphate synthase OPRT subunit complex==
-[[2wns]] - hOPRT + OMP