6z92: Difference between revisions
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==[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) solved by Fe-SAD phasing== | ==[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) solved by Fe-SAD phasing== | ||
<StructureSection load='6z92' size='340' side='right'caption='[[6z92]]' scene=''> | <StructureSection load='6z92' size='340' side='right'caption='[[6z92]], [[Resolution|resolution]] 2.07Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z92 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z92 FirstGlance]. <br> | <table><tr><td colspan='2'>[[6z92]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z92 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z92 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z92 OCA], [http://pdbe.org/6z92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z92 RCSB], [http://www.ebi.ac.uk/pdbsum/6z92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z92 ProSAT]</span></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z92 OCA], [http://pdbe.org/6z92 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z92 RCSB], [http://www.ebi.ac.uk/pdbsum/6z92 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z92 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We recently discovered a [Fe-S]-containing protein with in vivo thiouracil desulfidase activity called TudS. We report here the crystal structure of TudS, refined at 1.5 A resolution, which harbors a [4Fe-4S] cluster, bound by three cysteines only. Incubation of TudS crystals with 4-thiouracil trapped the cluster with a hydrosulfide ligand bound to the fourth non-protein-bonded iron, as established by the sulfur anomalous signal. This indicates that a [4Fe-5S] state of the cluster is a catalytic intermediate in the desulfuration reaction. Structural data and site-directed mutagenesis indicate that a water molecule is located next to the hydrosulfide ligand and to two catalytically important residues, Ser101 and Glu45. This information together with modeling studies allow us to propose a mechanism for the unprecedented nonredox enzymatic desulfuration of thiouracil, in which a [4Fe-4S] cluster binds and activates the sulfur atom of the substrate. | |||
Structural evidence for a [4Fe-5S] intermediate in the non-redox desulfuration of thiouracil.,Zhou J, Pecqueur L, Aucynaite A, Fuchs J, Rutkiene R, Vaitekunas J, Meskys R, Boll M, Fontecave M, Urbonavicius J, Golinelli-Pimpaneau B Angew Chem Int Ed Engl. 2020 Sep 15. doi: 10.1002/anie.202011211. PMID:32929873<ref>PMID:32929873</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6z92" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fontecave M]] | [[Category: Uncultivated bacterium]] | ||
[[Category: Golinelli-Pimpaneau B]] | [[Category: Fontecave, M]] | ||
[[Category: Pecqueur L]] | [[Category: Golinelli-Pimpaneau, B]] | ||
[[Category: Zhou J]] | [[Category: Pecqueur, L]] | ||
[[Category: Zhou, J]] | |||
[[Category: Desulfuration]] | |||
[[Category: Iron-sulfur cluster]] | |||
[[Category: Sulfurtransferase]] | |||
[[Category: Thiouracil]] | |||
[[Category: Transferase]] | |||