Transposase: Difference between revisions
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<StructureSection load='3ecp' size=' | <StructureSection load='3ecp' size='350' side='right' caption='Structure of Tn5 transposase complex with DNA and glycerol (PDB code [[3ecp]]).' scene='59/596996/Cv/8'> | ||
__TOC__ | __TOC__ | ||
== Function == | == Function == | ||
'''Transposases''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>. <br /> | '''Transposases''' or '''transposition proteins''' bind to the ends of a transposon and catalyze its movement to another part of the genome by a cut-and-paste mechanism<ref>PMID:19478801</ref>. <br /> | ||
*'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br /> | *'''Mu transposase''' (MuA) is essential for integration, replication-transposition and excision of bacteriophage Mu DNA into multiple sites of bacterial genome. Mu transposition occurs within the transposome which is a protein-DNA complex which includes 4 subunits of MuA<ref>PMID:7628012</ref>.<br /> | ||
*'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br /> | *'''Hermes transposase''' is a fly protein<ref>PMID:19450689</ref>.<br /> | ||
* '''Dra2 transposase''' is a ''Deinococcus radiodurans'' protein<br /> | * '''Dra2 transposase''' is a ''Deinococcus radiodurans'' protein<br /> | ||
*'''Tn5 transposase''' active site contains the DDE motif which catalyzes the movement of the transposon<ref>PMID:18680433</ref>.<br /> | *'''Tn5 transposase''' active site contains the DDE motif which catalyzes the movement of the transposon<ref>PMID:18680433</ref>.<br /> | ||
*'''Tn7 transposase''' is bacterial and highly specific<ref>PMID:1664019</ref>.<br /> | |||
*'''Mos1 transposase''' mediates the movement of Mos1 transposon<ref>PMID:10762422</ref><br /> | *'''Mos1 transposase''' mediates the movement of Mos1 transposon<ref>PMID:10762422</ref><br /> | ||
*'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<ref>PMID:7838706</ref><br /> | *'''Tc3 transposase''' mediates the movement of Tc3 transposon in ''C. elegans''<ref>PMID:7838706</ref><br /> | ||
*'''Sleeping beauty transposase''' synthetic transposon developed as a non-viral vector for gene therapy<ref>PMID:14632258</ref><br /> | |||
== Structural highlights == | == Structural highlights == | ||
Transposase contains a DDE motif in its active site<ref>PMID:18790806</ref> enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. The DDE motif is conserved in transposases and retroviral integrases and is essential for their activity. | <scene name='59/596996/Cv/9'>The Biological Assembly of Tn5 transposase is homodimer</scene> (PDB code [[3ecp]]). Transposase contains a <scene name='59/596996/Cv/7'>DDE motif in its active site</scene><ref>PMID:18790806</ref> enabling it to coordinate the binding of divalent metal ions. The metal ions affect transposase reactivity. The DDE motif is conserved in transposases and retroviral integrases and is essential for their activity. | ||
==3D structures of transposase== | ==3D structures of transposase== | ||
[[Transposase 3D structures]] | |||
</StructureSection> | |||
== References == | == References == | ||