Tryptophan RNA-binding attenuation protein: Difference between revisions

Latest revision as of 13:31, 21 December 2020

Function

Tryptophan RNA-binding attenuation protein (TRAP) or transcription attenuation protein MtrB is a tryptophan-activated RNA-binding protein which regulates the expression of the tryptophan biosynthesis genes. Down regulation of Trp biosynthesis is observed upon binding of 11 tryptophan molecules to TRAP^[1].

Structural highlights

TRAP is composed of 11 identical subunits arranged in a . The ^[2]. Water molecules are shown as red spheres.

Structure of tryptophan RNA-binding attenuation protein complex with Trp (PDB code 2exs).

Drag the structure with the mouse to rotate

3D Structures of tryptophan RNA-binding attenuation protein3D Structures of tryptophan RNA-binding attenuation protein

Updated on 21-December-2020

ReferencesReferences

↑ Li PT, Gollnick P. Characterization of a trp RNA-binding attenuation protein (TRAP) mutant with tryptophan independent RNA binding activity. J Mol Biol. 2004 Jan 16;335(3):707-22. PMID:14687568 doi:http://dx.doi.org/10.1016/S0022283603013846
↑ Heddle JG, Yokoyama T, Yamashita I, Park SY, Tame JR. Rounding up: Engineering 12-membered rings from the cyclic 11-mer TRAP. Structure. 2006 May;14(5):925-33. PMID:16698553 doi:http://dx.doi.org/10.1016/j.str.2006.03.013

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Li PT, Gollnick P. Characterization of a trp RNA-binding attenuation protein (TRAP) mutant with tryptophan independent RNA binding activity. J Mol Biol. 2004 Jan 16;335(3):707-22. PMID:14687568 doi:http://dx.doi.org/10.1016/S0022283603013846

[2] Heddle JG, Yokoyama T, Yamashita I, Park SY, Tame JR. Rounding up: Engineering 12-membered rings from the cyclic 11-mer TRAP. Structure. 2006 May;14(5):925-33. PMID:16698553 doi:http://dx.doi.org/10.1016/j.str.2006.03.013

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@@ Line 1: / Line 1: @@
-<StructureSection load='1utf' size='340' side='right' caption='Structure of tryptophan RNA-binding attenuation protein complex with RNA and Trp (PDB code [[1utf]]).' scene=''>
+<StructureSection load='2exs' size='350' side='right' caption='Structure of tryptophan RNA-binding attenuation protein complex with Trp (PDB code [[2exs]]).' scene=''>
 == Function ==
-'''Tryptophan RNA-binding attenuation protein''' (TRAP) is a tryptophan-activated RNA-binding protein which regulates the expression of the tryptophan biosynthesis genes.  TRAP is composed of 11 identical subunits arranged in a symmetrical ring.  Down regulation of Trp biosynthesis is observed upon binding of 11 Trp to TRAP.
+'''Tryptophan RNA-binding attenuation protein''' (TRAP) or '''transcription attenuation protein MtrB''' is a tryptophan-activated RNA-binding protein which regulates the expression of the tryptophan biosynthesis genes.  Down regulation of Trp biosynthesis is observed upon binding of 11 tryptophan molecules to TRAP<ref>PMID:14687568</ref>.
-== Disease ==
-== Relevance ==
 == Structural highlights ==
+TRAP is composed of 11 identical subunits arranged in a <scene name='59/595823/Cv/4'>symmetrical ring</scene>. The <scene name='59/595823/Cv/3'>active site of TRAP</scene><ref>PMID:16698553</ref>. Water molecules are shown as red spheres.
+</StructureSection>
 == 3D Structures of tryptophan RNA-binding attenuation protein ==
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
-===TRAP===
+*Tryptophan RNA-binding attenuation protein
-[[3aqd]] – GsTRAP – ''Geobacillus stearothermophilus''<br />
-===TRAP complex with Trp===
+**[[3aqd]] – GsTRAP – ''Geobacillus stearothermophilus''<br />
-[[2zdo]], [[2exs]], [[2ext]], [[2zcz]], [[2zd0]] – GsTRAP + Trp<br />
+*Tryptophan RNA-binding attenuation protein binary complex with Trp
-[[1wap]], [[3zzq]] – BsTRAP + Trp – ''Bacillus subtilis''<br />
-[[4b27]] – BsTRAP (mutant) + Trp <br />
-[[3zte]] – TRAP + Trp – ''Bacillus licheniformis''<br />
-[[3zzl]] – TRAP + Trp – ''Bacillus halodurans''<br />
-===TRAP ternary complex with Trp===
+**[[2zdo]], [[2exs]], [[2ext]], [[2zcz]], [[2zd0]], [[3zzs]], [[1qaw]] – GsTRAP + Trp<br />
+**[[5eew]], [[5eex]], [[5eey]], [[5eez]], [[5ef0]], [[5ef1]], [[5ef2]], [[5ef3]]  – GsTRAP + Trp – radiation damage<br />
+**[[1wap]], [[3zzq]] – BsTRAP + Trp – ''Bacillus subtilis''<br />
+**[[4b27]] – BsTRAP (mutant) + Trp <br />
+**[[3zte]] – TRAP + Trp – ''Bacillus licheniformis''<br />
+**[[3zzl]] – TRAP + Trp – ''Bacillus halodurans''<br />
-[[1c9s]], [[1gtf]], [[1gtn]], [[1utd]], [[1utf]], [[1utv]] – GsTRAP + RNA + Trp <br />
+*Tryptophan RNA-binding attenuation protein ternary complex with Trp
-[[2zp8]], [[2zp9]] – GsTRAP + TRAP inhibitory protein + Trp <br />
+**[[1c9s]], [[1gtf]], [[1gtn]], [[1utd]], [[4v4f]] – GsTRAP + RNA + Trp <br />
+**[[2zp8]], [[2zp9]] – GsTRAP + TRAP inhibitory protein + Trp <br />
+}}
 == References ==
 <references/>
 [[Category:Topic Page]]