6qy3: Difference between revisions

Latest revision as of 08:12, 11 April 2020

Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas systemSegment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system

6qy3, resolution 9.10Å

Structural highlights

6qy3 is a 54 chain structure with sequence from Atcc 19258 and Escherichia coli bl21(de3). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	6qxf, 6qxt
Gene:	csn2 (ATCC 19258), cas1 (ATCC 19258), cas2 (ATCC 19258)
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

CRISPR and associated Cas proteins function as an adaptive immune system in prokaryotes to combat bacteriophage infection. During the immunization step, new spacers are acquired by the CRISPR machinery, but the molecular mechanism of spacer capture remains enigmatic. We show that the Cas9, Cas1, Cas2, and Csn2 proteins of a Streptococcus thermophilus type II-A CRISPR-Cas system form a complex and provide cryoelectron microscopy (cryo-EM) structures of three different assemblies. The predominant form, with the stoichiometry Cas18-Cas24-Csn28, referred to as monomer, contains approximately 30 bp duplex DNA bound along a central channel. A minor species, termed a dimer, comprises two monomers that sandwich a further eight Cas1 and four Cas2 subunits and contains two DNA approximately 30-bp duplexes within the channel. A filamentous form also comprises Cas18-Cas24-Csn28 units (typically 2-6) but with a different Cas1-Cas2 interface between them and a continuous DNA duplex running along a central channel.

Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System.,Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB Mol Cell. 2019 May 6. pii: S1097-2765(19)30312-0. doi:, 10.1016/j.molcel.2019.04.020. PMID:31080012^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB. Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System. Mol Cell. 2019 May 6. pii: S1097-2765(19)30312-0. doi:, 10.1016/j.molcel.2019.04.020. PMID:31080012 doi:http://dx.doi.org/10.1016/j.molcel.2019.04.020

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OCA

[1] Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB. Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System. Mol Cell. 2019 May 6. pii: S1097-2765(19)30312-0. doi:, 10.1016/j.molcel.2019.04.020. PMID:31080012 doi:http://dx.doi.org/10.1016/j.molcel.2019.04.020

[1]

@@ Line 3: / Line 3: @@
 <SX load='6qy3' size='340' side='right' viewer='molstar' caption='[[6qy3]], [[Resolution|resolution]] 9.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6qy3]] is a 54 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19258 Atcc 19258] and [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QY3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6qy3]] is a 54 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19258 Atcc 19258] and [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QY3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QY3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6qxf|6qxf]], [[6qxt|6qxt]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">csn2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1308 ATCC 19258]), cas1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1308 ATCC 19258]), cas2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1308 ATCC 19258])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qy3 OCA], [http://pdbe.org/6qy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6qy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qy3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qy3 OCA], [http://pdbe.org/6qy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6qy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qy3 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

6qy3: Difference between revisions

Latest revision as of 08:12, 11 April 2020

Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas systemSegment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

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6qy3: Difference between revisions

Latest revision as of 08:12, 11 April 2020

Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas systemSegment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system

Structural highlights

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

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