6hy0: Difference between revisions

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-'''Unreleased structure'''
-The entry 6hy0 is ON HOLD  until Paper Publication
+==Atomic models of P1, P4 C-terminal fragment and P8 fitted in the bacteriophage phi6 nucleocapsid reconstructed with icosahedral symmetry==
+<SX load='6hy0' size='340' side='right' viewer='molstar' caption='[[6hy0]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6hy0]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HY0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HY0 FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hy0 OCA], [http://pdbe.org/6hy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6hy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hy0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/P1_BPPH6 P1_BPPH6]] P1 is the major inner capsid (core) protein of the polyhedral procapsid, which is responsible for genomic replication and transcription. Forms a dodecahedral shell from 60 asymmetric dimers. Binds to RNA and may be involved in genomic packaging.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Characterizing the genome of mature virions is pivotal to understanding the highly dynamic processes of virus assembly and infection. Owing to the different cellular fates of DNA and RNA, the life cycles of double-stranded (ds)DNA and dsRNA viruses are dissimilar. In terms of nucleic acid packing, dsDNA viruses, which lack genome segmentation and intra-capsid transcriptional machinery, predominantly display single-spooled genome organizations(1-8). Because the release of dsRNA into the cytoplasm triggers host defence mechanisms(9), dsRNA viruses retain their genomes within a core particle that contains the enzymes required for RNA replication and transcription(10-12). The genomes of dsRNA viruses vary greatly in the degree of segmentation. In members of the Reoviridae family, genomes consist of 10-12 segments and exhibit a non-spooled arrangement mediated by RNA-dependent RNA polymerases(11-14). However, whether this arrangement is a general feature of dsRNA viruses remains unknown. Here, using cryo-electron microscopy to resolve the dsRNA genome structure of the tri-segmented bacteriophage 6 of the Cystoviridae family, we show that dsRNA viruses can adopt a dsDNA-like single-spooled genome organization. We find that in this group of viruses, RNA-dependent RNA polymerases do not direct genome ordering, and the dsRNA can adopt multiple conformations. We build a model that encompasses 90% of the genome, and use this to quantify variation in the packing density and to characterize the different liquid crystalline geometries that are exhibited by the tightly compacted nucleic acid. Our results demonstrate that the canonical model for the packing of dsDNA can be extended to dsRNA viruses.
-Authors: El Omari, K., Ilca, S.L., Stuart, D.I., Huiskonen, J.T.
+Multiple liquid crystalline geometries of highly compacted nucleic acid in a dsRNA virus.,Ilca SL, Sun X, El Omari K, Kotecha A, de Haas F, DiMaio F, Grimes JM, Stuart DI, Poranen MM, Huiskonen JT Nature. 2019 May 29. pii: 10.1038/s41586-019-1229-9. doi:, 10.1038/s41586-019-1229-9. PMID:31142835<ref>PMID:31142835</ref>
-Description: Atomic models of P1, P4 C-terminal fragment and P8 fitted in the bacteriophage phi6 nucleocapsid reconstructed with icosahedral symmetry
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: El Omari, K]]
+<div class="pdbe-citations 6hy0" style="background-color:#fffaf0;"></div>
-[[Category: Ilca, S.L]]
+== References ==
-[[Category: Huiskonen, J.T]]
+<references/>
-[[Category: Stuart, D.I]]
+__TOC__
+</SX>
+[[Category: Large Structures]]
+[[Category: Pseudomonas phage phi6]]
+[[Category: Huiskonen, J T]]
+[[Category: Ilca, S L]]
+[[Category: Omari, K El]]
+[[Category: Stuart, D I]]
+[[Category: Capsid]]
+[[Category: Dsrna]]
+[[Category: Virus]]