6c3p: Difference between revisions
New page: '''Unreleased structure''' The entry 6c3p is ON HOLD Authors: Lee, K.P.K., Chen, J., MacKinnon, R. Description: Cryo-EM structure of human KATP bound to ATP and ADP in propeller form [... |
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The entry | ==Cryo-EM structure of human KATP bound to ATP and ADP in propeller form== | ||
<SX load='6c3p' size='340' side='right' viewer='molstar' caption='[[6c3p]], [[Resolution|resolution]] 5.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6c3p]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6C3P FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6c3o|6c3o]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6c3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c3p OCA], [http://pdbe.org/6c3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c3p RCSB], [http://www.ebi.ac.uk/pdbsum/6c3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c3p ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[[http://www.uniprot.org/uniprot/KCJ11_HUMAN KCJ11_HUMAN]] MODY;Autosomal dominant hyperinsulinism due to Kir6.2 deficiency;Intermediate DEND syndrome;Transient neonatal diabetes mellitus;Permanent neonatal diabetes mellitus;DEND syndrome;Diazoxide-resistant focal hyperinsulinism due to Kir6.2 deficiency;Autosomal recessive hyperinsulinism due to Kir6.2 deficiency. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. Defects in KCNJ11 may contribute to non-insulin-dependent diabetes mellitus (NIDDM), also known as diabetes mellitus type 2. The disease is caused by mutations affecting the gene represented in this entry. [[http://www.uniprot.org/uniprot/ABCC8_HUMAN ABCC8_HUMAN]] Diazoxide-resistant focal hyperinsulinism due to SUR1 deficiency;Autosomal dominant hyperinsulinism due to SUR1 deficiency;Transient neonatal diabetes mellitus;DEND syndrome;Autosomal recessive hyperinsulinism due to SUR1 deficiency;MODY;Permanent neonatal diabetes mellitus. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/KCJ11_HUMAN KCJ11_HUMAN]] This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity). Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.<ref>PMID:17855752</ref> <ref>PMID:28842488</ref> <ref>PMID:9831708</ref> [[http://www.uniprot.org/uniprot/ABCC8_HUMAN ABCC8_HUMAN]] Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release.<ref>PMID:24814349</ref> <ref>PMID:25720052</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In many excitable cells KATP channels respond to intracellular adenosine nucleotides: ATP inhibits while ADP activates. We present two structures of the human pancreatic KATP channel, containing the ABC transporter SUR1 and the inward-rectifier K(+) channel Kir6.2, in the presence of Mg(2+) and nucleotides. These structures, referred to as quatrefoil and propeller forms, were determined by single-particle cryo-EM at 3.9 A and 5.6 A, respectively. In both forms ATP occupies the inhibitory site in Kir6.2. The nucleotide-binding domains of SUR1 are dimerized with Mg(2+)-ATP in the degenerate site and Mg(2+)-ADP in the consensus site. A lasso extension forms an interface between SUR1 and Kir6.2 adjacent to the ATP site in the propeller form, and is disrupted in the quatrefoil form. These structures support the role of SUR1 as an ADP sensor and highlight the lasso extension as a key regulatory element in ADP's ability to override ATP inhibition. | |||
Molecular structure of human KATP in complex with ATP and ADP.,Lee KPK, Chen J, MacKinnon R Elife. 2017 Dec 29;6. doi: 10.7554/eLife.32481. PMID:29286281<ref>PMID:29286281</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6c3p" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Potassium channel 3D structures|Potassium channel 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Chen, J]] | [[Category: Chen, J]] | ||
[[Category: Lee, K | [[Category: Lee, K P.K]] | ||
[[Category: | [[Category: MacKinnon, R]] | ||
[[Category: Atp-dependent potassium channel kir6 2 sur1 atp adp]] | |||
[[Category: Transport protein]] | |||