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==Cryo-EM reconstruction of the maedi-visna virus (MVV) intasome== | |||
<SX load='5m0q' size='340' side='right' viewer='molstar' caption='[[5m0q]], [[Resolution|resolution]] 4.91Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5m0q]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Mvv Mvv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M0Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M0Q FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36374 MVV])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m0q OCA], [http://pdbe.org/5m0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m0q RCSB], [http://www.ebi.ac.uk/pdbsum/5m0q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m0q ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/POL_VILVK POL_VILVK]] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Retroviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze insertion of viral DNA into cellular chromatin. Using cryo-electron microscopy, we now visualize the functional maedi-visna lentivirus intasome at 4.9 angstrom resolution. The intasome comprises a homo-hexadecamer of IN with a tetramer-of-tetramers architecture featuring eight structurally distinct types of IN protomers supporting two catalytically competent subunits. The conserved intasomal core, previously observed in simpler retroviral systems, is formed between two IN tetramers, with a pair of C-terminal domains from flanking tetramers completing the synaptic interface. Our results explain how HIV-1 IN, which self-associates into higher-order multimers, can form a functional intasome, reconcile the bulk of early HIV-1 IN biochemical and structural data, and provide a lentiviral platform for design of HIV-1 IN inhibitors. | |||
A supramolecular assembly mediates lentiviral DNA integration.,Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P Science. 2017 Jan 6;355(6320):93-95. doi: 10.1126/science.aah7002. PMID:28059770<ref>PMID:28059770</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5m0q" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Mvv]] | |||
[[Category: Ballandras-Colas, A]] | [[Category: Ballandras-Colas, A]] | ||
[[Category: | [[Category: Cherepanov, P]] | ||
[[Category: Costa, A]] | |||
[[Category: Kotecha, A]] | |||
[[Category: Locke, J]] | [[Category: Locke, J]] | ||
[[Category: Maskell, D]] | [[Category: Maskell, D]] | ||
[[Category: | [[Category: Pye, V E]] | ||
[[Category: Swuec, S]] | |||
[[Category: Dna-binding]] | |||
[[Category: Integrase]] | |||
[[Category: Lentivirus]] | |||
[[Category: Retrovirus]] | |||
[[Category: Rnaseh fold]] | |||
[[Category: Viral protein]] | |||
[[Category: Zn-binding]] | |||
Latest revision as of 23:50, 10 April 2020
Cryo-EM reconstruction of the maedi-visna virus (MVV) intasomeCryo-EM reconstruction of the maedi-visna virus (MVV) intasome
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