3los: Difference between revisions
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==Atomic Model of Mm-cpn in the Closed State== | |||
<SX load='3los' size='340' side='right' viewer='molstar' caption='[[3los]], [[Resolution|resolution]] 4.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3los]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43000 Atcc 43000]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3iye 3iye]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LOS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3LOS FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3iyf|3iyf]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hsp60, MMP1515 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39152 ATCC 43000])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3los FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3los OCA], [http://pdbe.org/3los PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3los RCSB], [http://www.ebi.ac.uk/pdbsum/3los PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3los ProSAT]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lo/3los_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3los ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Group II chaperonins are essential mediators of cellular protein folding in eukaryotes and archaea. These oligomeric protein machines, approximately 1 megadalton, consist of two back-to-back rings encompassing a central cavity that accommodates polypeptide substrates. Chaperonin-mediated protein folding is critically dependent on the closure of a built-in lid, which is triggered by ATP hydrolysis. The structural rearrangements and molecular events leading to lid closure are still unknown. Here we report four single particle cryo-electron microscopy (cryo-EM) structures of Mm-cpn, an archaeal group II chaperonin, in the nucleotide-free (open) and nucleotide-induced (closed) states. The 4.3 A resolution of the closed conformation allowed building of the first ever atomic model directly from the single particle cryo-EM density map, in which we were able to visualize the nucleotide and more than 70% of the side chains. The model of the open conformation was obtained by using the deformable elastic network modelling with the 8 A resolution open-state cryo-EM density restraints. Together, the open and closed structures show how local conformational changes triggered by ATP hydrolysis lead to an alteration of intersubunit contacts within and across the rings, ultimately causing a rocking motion that closes the ring. Our analyses show that there is an intricate and unforeseen set of interactions controlling allosteric communication and inter-ring signalling, driving the conformational cycle of group II chaperonins. Beyond this, we anticipate that our methodology of combining single particle cryo-EM and computational modelling will become a powerful tool in the determination of atomic details involved in the dynamic processes of macromolecular machines in solution. | |||
Mechanism of folding chamber closure in a group II chaperonin.,Zhang J, Baker ML, Schroder GF, Douglas NR, Reissmann S, Jakana J, Dougherty M, Fu CJ, Levitt M, Ludtke SJ, Frydman J, Chiu W Nature. 2010 Jan 21;463(7279):379-83. PMID:20090755<ref>PMID:20090755</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3los" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Chaperonin 3D structures|Chaperonin 3D structures]] | |||
*[[Heat Shock Protein structures|Heat Shock Protein structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Atcc 43000]] | |||
[[Category: Large Structures]] | |||
[[Category: Baker, M L]] | |||
[[Category: Chiu, W]] | |||
[[Category: Dougherty, M]] | |||
[[Category: Douglas, N R]] | |||
[[Category: Frydman, J]] | |||
[[Category: Fu, C J]] | |||
[[Category: Jakana, J]] | |||
[[Category: Levitt, M]] | |||
[[Category: Ludtke, S J]] | |||
[[Category: Reissmann, S]] | |||
[[Category: Schroeder, G]] | |||
[[Category: Zhang, J]] | |||
[[Category: Atp-binding]] | |||
[[Category: Chaperone]] | |||
[[Category: Group ii chaperonin]] | |||
[[Category: Methanococcus maripaludi]] | |||
[[Category: Mm-cpn]] | |||
[[Category: Nucleotide-binding]] | |||
[[Category: Protein folding]] | |||
[[Category: Single particle reconstruction]] | |||