TolA: Difference between revisions
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<StructureSection load='1lr0' size='350' side='right' scene='' caption='TolA domain III complex with TRIS and Zn+2 ion (grey) (PDB code [[1lr0]])'> | |||
==Structure== | ==Structure== | ||
TolA is located in the inner membrane and comprises of three domains: the N-terminal domain I (TolAI), from residues 1-47 including a 20-residue hydrophobic membrane spanning region which anchors the protein to the cytoplasmic membrane<ref>PMID: 7853390</ref>; domain II (TolAII), from residues 48-301, which forms a rigid helix connecting the domains either side of it; and the C-terminal domain III (TolAIII) from residues 302-421, which may be involved in the function of TolA by interacting with the periplasmic or outer membrane proteins, due to the tethering to domain II<ref name='Sharyn'>PMID: 8416897</ref>. | '''TolA''' is located in the inner membrane and comprises of three domains: the N-terminal domain I (TolAI), from residues 1-47 including a 20-residue hydrophobic membrane spanning region which anchors the protein to the cytoplasmic membrane<ref name='Lazzaroni'>PMID: 7853390</ref>; domain II (TolAII), from residues 48-301, which forms a rigid helix connecting the domains either side of it; and the C-terminal domain III (TolAIII) from residues 302-421, which may be involved in the function of TolA by interacting with the periplasmic or outer membrane proteins, due to the tethering to domain II<ref name='Sharyn'>PMID: 8416897</ref>. For additional details see [[Tol]]. | ||
===C-terminal Domain=== | ===C-terminal Domain=== | ||
The C-terminal domain of TolA is directly involved with the N-terminal of both Colicin and the phage minor coat gene 3 protein<ref>PMID: 15701516</ref>. | The C-terminal domain of TolA is directly involved with the N-terminal of both Colicin and the phage minor coat gene 3 protein<ref name='Deprez'>PMID: 15701516</ref>. | ||
===Comparison with TonB=== | ===Comparison with TonB=== | ||
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Although the exact function of TolA is not yet known, it has been shown that unlike mutations in the proteins of the TonB system to which the TolQRA proteins show many similarities, mutations in the TolQRA proteins affect the outer membrane integrity. TolA could be involved structurally by bringing the inner and outer membranes together and forming a bridge or link between them<ref name='Sharyn'>PMID: 8416897</ref>. | Although the exact function of TolA is not yet known, it has been shown that unlike mutations in the proteins of the TonB system to which the TolQRA proteins show many similarities, mutations in the TolQRA proteins affect the outer membrane integrity. TolA could be involved structurally by bringing the inner and outer membranes together and forming a bridge or link between them<ref name='Sharyn'>PMID: 8416897</ref>. | ||
TolA plays an important role in the import mechanisms for the uptake of bacteriotoxins (see [[Colicin]]) and the DNA of filamentous bacteriophages<ref name='Deprez'>PMID: 15701516</ref>, and has also been shown to be involved in the bacterial sensitivity to these groups<ref>PMID: 7853390</ref>, in particular the alpha helix of domain II, the deletion of which causes increased cellular sensitivity to deoxycholate (a detergent)<ref name='Schendel | TolA plays an important role in the import mechanisms for the uptake of bacteriotoxins (see [[Colicin]]) and the DNA of filamentous bacteriophages<ref name='Deprez'>PMID: 15701516</ref>, and has also been shown to be involved in the bacterial sensitivity to these groups<ref name='Lazzaroni'>PMID: 7853390</ref>, in particular the alpha helix of domain II, the deletion of which causes increased cellular sensitivity to deoxycholate (a detergent)<ref name='Schendel'>PMID: 9171417</ref>. | ||
===TolA and Colicin Uptake=== | ===TolA and Colicin Uptake=== | ||
Studies have shown that TolAIII plays an important role in the uptake mechanisms of colicin and DNA by being directly involved with the N-terminal of both colicin and the phage minor coat gene 3 protein<ref>PMID: 15701516</ref>. | Studies have shown that TolAIII plays an important role in the uptake mechanisms of colicin and DNA by being directly involved with the N-terminal of both colicin and the phage minor coat gene 3 protein<ref name='Deprez'>PMID: 15701516</ref>. | ||
TolA interacts with different group A colicins in different ways<ref name='Schendel | TolA interacts with different group A colicins in different ways<ref name='Schendel'>PMID: 9171417</ref>: | ||
* [[Colicin E1]] requires different structural features of TolA to be transported than the other group A colicins, including [[Colicin E3]], [[Colicin N]] and [[Colicin A]]. Colicin E3 requires the C-terminus of TolA, which it finds with ease even when the terminus is attached very close to the membrane-spanning domain, by means of utilising a different outer membrane receptor complex to that of the other colicins | * [[Colicin E1]] requires different structural features of TolA to be transported than the other group A colicins, including [[Colicin E3]], [[Colicin N]] and [[Colicin A]]. Colicin E3 requires the C-terminus of TolA, which it finds with ease even when the terminus is attached very close to the membrane-spanning domain, by means of utilising a different outer membrane receptor complex to that of the other colicins | ||
* The other colicins require additional receptors in order to bind initially to the outer membrane | * The other colicins require additional receptors in order to bind initially to the outer membrane | ||
*Colicin E1 also does not require as high of TolA levels than the other colicins in order for translocation to occur | *Colicin E1 also does not require as high of TolA levels than the other colicins in order for translocation to occur | ||
</StructureSection> | |||
==3D structures of TolA== | |||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | |||
[[1lr0]] – TolA domain III – ''Pseudomonas aeruginosa''<br /> | |||
[[1s62]] – EcTolA C terminal – ''Escherichia coli'' – NMR<br /> | |||
[[2x9a]] - EcTolA C terminal + attachment protein G3P<br /> | |||
[[1tol]] - EcTolA C terminal/minor coat protein<br /> | |||
[[3qdr]] - EcTolA domain III + colicin A residues 53-107<br /> | |||
[[3qdp]] - EcTolA domain III <br /> | |||
[[6fw4]] – VcTolA C terminal – ''Vibrio cholerae'' - NMR<br /> | |||
[[4g7x]] – VcTolA C terminal + uncharacterized protein<br /> | |||
==References== | ==References== | ||
<references/> | <references/> | ||
[[Category: Topic Page]] | |||