6d7g: Difference between revisions
New page: '''Unreleased structure''' The entry 6d7g is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Structure of 5F3 TCR in complex with HLA-A2/MART-1== | |||
<StructureSection load='6d7g' size='340' side='right'caption='[[6d7g]], [[Resolution|resolution]] 2.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6d7g]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D7G FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HLA-A, HLAA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRA@ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRGV8, TRGC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d7g OCA], [http://pdbe.org/6d7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d7g RCSB], [http://www.ebi.ac.uk/pdbsum/6d7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d7g ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Antigen recognition by T cells bearing alphabeta T cell receptors (TCRs) is restricted by major histocompatibility complex (MHC). However, how antigens are recognized by T cells bearing gammadelta TCRs remains unclear. Although gammadelta T cells can recognize nonclassical MHC, it is generally thought that recognition of antigens is not MHC restricted. Here, we took advantage of an in vitro system to generate antigen-specific human T cells and show that melanoma-associated antigens, MART-1 and gp100, can be recognized by gammadelta T cells in an MHC-restricted fashion. Cloning and transferring of MART-1-specific gammadelta TCRs restored the specific recognition of the initial antigen MHC/peptide reactivity and conferred antigen-specific functional responses. A crystal structure of a MART-1-specific gammadelta TCR, together with MHC/peptide, revealed distinctive but similar docking properties to those previously reported for alphabeta TCRs, recognizing MART-1 on HLA-A*0201. Our work shows that antigen-specific and MHC-restricted gammadelta T cells can be generated in vitro and that MART-1-specific gammadelta T cells can also be found and cloned from the naive repertoire. These findings reveal that classical MHC-restricted human gammadelta TCRs exist in the periphery and have the potential to be used in developing of new TCR-based immunotherapeutic approaches. | |||
Generation and molecular recognition of melanoma-associated antigen-specific human gammadelta T cells.,Benveniste PM, Roy S, Nakatsugawa M, Chen ELY, Nguyen L, Millar DG, Ohashi PS, Hirano N, Adams EJ, Zuniga-Pflucker JC Sci Immunol. 2018 Dec 14;3(30). pii: 3/30/eaav4036. doi:, 10.1126/sciimmunol.aav4036. PMID:30552102<ref>PMID:30552102</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6d7g" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Human]] | |||
[[Category: Large Structures]] | |||
[[Category: Adams, E J]] | |||
[[Category: Roy, S]] | |||
[[Category: Complex]] | |||
[[Category: Immune system]] | |||
[[Category: Mhc]] | |||
[[Category: Peptide recognition]] | |||
[[Category: Tcr]] | |||
Latest revision as of 13:17, 26 February 2020
Structure of 5F3 TCR in complex with HLA-A2/MART-1Structure of 5F3 TCR in complex with HLA-A2/MART-1
Structural highlights
Publication Abstract from PubMedAntigen recognition by T cells bearing alphabeta T cell receptors (TCRs) is restricted by major histocompatibility complex (MHC). However, how antigens are recognized by T cells bearing gammadelta TCRs remains unclear. Although gammadelta T cells can recognize nonclassical MHC, it is generally thought that recognition of antigens is not MHC restricted. Here, we took advantage of an in vitro system to generate antigen-specific human T cells and show that melanoma-associated antigens, MART-1 and gp100, can be recognized by gammadelta T cells in an MHC-restricted fashion. Cloning and transferring of MART-1-specific gammadelta TCRs restored the specific recognition of the initial antigen MHC/peptide reactivity and conferred antigen-specific functional responses. A crystal structure of a MART-1-specific gammadelta TCR, together with MHC/peptide, revealed distinctive but similar docking properties to those previously reported for alphabeta TCRs, recognizing MART-1 on HLA-A*0201. Our work shows that antigen-specific and MHC-restricted gammadelta T cells can be generated in vitro and that MART-1-specific gammadelta T cells can also be found and cloned from the naive repertoire. These findings reveal that classical MHC-restricted human gammadelta TCRs exist in the periphery and have the potential to be used in developing of new TCR-based immunotherapeutic approaches. Generation and molecular recognition of melanoma-associated antigen-specific human gammadelta T cells.,Benveniste PM, Roy S, Nakatsugawa M, Chen ELY, Nguyen L, Millar DG, Ohashi PS, Hirano N, Adams EJ, Zuniga-Pflucker JC Sci Immunol. 2018 Dec 14;3(30). pii: 3/30/eaav4036. doi:, 10.1126/sciimmunol.aav4036. PMID:30552102[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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