Selenomethionine: Difference between revisions

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Eric Martz

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	Selenomethionine is methionine in which the sulfur atom is replaced by selenium. Methionine is one of the [[amino acids\|20 standard amino acids]] (see [https://en.wikipedia.org/wiki/Methionine Methionine in Wikipedia]). Selenomethionine occurs naturally (see [https://en.wikipedia.org/wiki/Selenomethionine Selenomethionine in Wikipedia) but is also introduced artificially into proteins prior to crystallization in order to solve phases by multi-wavelength anomalous dispersion (MAD; see [https://en.wikipedia.org/wiki/Multi-wavelength_anomalous_dispersion MAD in Wikipedia).		Selenomethionine (designated MSE in the [[Protein Data Bank]]) is methionine in which the sulfur atom is replaced by selenium. Methionine is one of the [[amino acids\|22 standard amino acids]] (see [https://en.wikipedia.org/wiki/Methionine Methionine in Wikipedia]). Selenomethionine occurs naturally (see [https://en.wikipedia.org/wiki/Selenomethionine Selenomethionine in Wikipedia]) but is also introduced artificially into proteins prior to crystallization in order to solve phases by multi-wavelength anomalous dispersion (MAD; see [https://en.wikipedia.org/wiki/Multi-wavelength_anomalous_dispersion MAD in Wikipedia]).

			[[4hik]] is an example of a crystallographic structure solved by MAD. The crystallized protein contained 143 amino acids taken from the middle of the 555 full-length protein. The wild-type sequence of the crystallized fragment contained three methionines, all of which were replaced by selenomethionine.