User:Nikhil Malvankar/Cytochrome nanowires: Difference between revisions
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[[Interactive_3D_Complement_in_Proteopedia|Interactive 3D Complement in Proteopedia]]<br> | [[Interactive_3D_Complement_in_Proteopedia|Interactive 3D Complement in Proteopedia]]<br> | ||
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<span style="font-size:160%"><b>Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers<ref name="m3" />.</b></span>< | <span style="font-size:160%"><b>Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers<ref name="m3" />.</b></span> | ||
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<span style="font-size:120%"> | <span style="font-size:120%"> | ||
Fengbin '''Wang''', | Fengbin '''Wang''', | ||
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April 4, 2019. [http://doi.org/10.1016/j.cell.2019.03.029 doi:10.1016/j.cell.2019.03.029] | April 4, 2019. [http://doi.org/10.1016/j.cell.2019.03.029 doi:10.1016/j.cell.2019.03.029] | ||
</span> | </span> | ||
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==Structure Tour== | ==Structure Tour== | ||
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===Nanowire Structure=== | ===Nanowire Structure=== | ||
<center>{{Template:Green links zoom}}</center> | |||
A nanowire model composed of 7 OmcS protein chains, each shown a different color, was constructed from the 3.2-3.7 Å cryo-EM density (<scene name='83/835223/Filament/1'>restore initial scene</scene>). The filament is ~4 nm in diamater, and has a characteristic undulating or sinusoidal form with a wavelength (pitch) of ~20 nm. The OmcS monomers have 407 amino acids each. The <scene name='83/835223/Filament/4'>carboxy terminus of each monomer contacts the amino terminus of the next</scene>. | A nanowire model composed of 7 OmcS protein chains, each shown a different color, was constructed from the 3.2-3.7 Å cryo-EM density (<scene name='83/835223/Filament/1'>restore initial scene</scene>). The filament is ~4 nm in diamater, and has a characteristic undulating or sinusoidal form with a wavelength (pitch) of ~20 nm. The OmcS monomers have 407 amino acids each. The <scene name='83/835223/Filament/4'>carboxy terminus of each monomer contacts the amino terminus of the next</scene>. | ||
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===Salt Bridges=== | ===Salt Bridges=== | ||
Using a 4.0 Å cutoff, [[6ef8]] has 7 salt bridges between amino acid sidechains (not shown). One of these, Arg176 to Asp432, is between protein chains, further strengthening the interfaces between monomers in the filament. | Using a 4.0 Å cutoff, [[6ef8]] has 7 salt bridges between amino acid sidechains (not shown). One of these, <scene name='83/835223/Inter-chain_salt_bridge/2'>Arg176 to Asp432 (2.6 Å)</scene> (<font color="#6070cf">'''Chain A'''</font>, <font color="#40af58">'''Chain B'''</font>, | ||
{{Template:ColorKey_Element_O}}, | |||
{{Template:ColorKey_Element_N}}), | |||
is between protein chains, further strengthening the interfaces between monomers in the filament. (These opposing charges are 4.9 Å apart in [[6nef]].) | |||
The amino-terminal NH<sup>3</sup>+ on Phe 1 forms a salt bridge with one carboxy of heme 2 (HEC503; 3.65 Å; not shown). | The amino-terminal NH<sup>3</sup>+ on Phe 1 forms a salt bridge with one carboxy of heme 2 (HEC503; 3.65 Å; not shown). | ||
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<br> | <br> | ||
<hr><br> | <hr> | ||
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==See Also== | ==See Also== | ||