Succinyl-CoA synthetase: Difference between revisions
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<StructureSection load='1cqj' size='350' side='right' scene='' caption='E. coli succinyl-CoA synthetase α subunit (grey, yellow) and β subunit (green, magenta) complex with CoA and phosphate (PDB code [[1cqj]])'> | <StructureSection load='1cqj' size='350' side='right' scene='' caption='E. coli succinyl-CoA synthetase α subunit (grey, yellow) and β subunit (green, magenta) complex with CoA and phosphate (PDB code [[1cqj]])'> | ||
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==Function== | |||
'''Succinyl-CoA synthetase''' or '''succinate-CoA ligase''' catalyzes the reversible reaction of succinyl-CoA + NDP + Pi <-> succinate + CoA + NTP (where N is either adenosine or guanosine. It can be found in Escherichia coli and is the fifth step in the [[Citric Acid Cycle|citric acid cycle]]. Due to its involvement in the citric acid cycle it is very important in all living cells that use oxygen as a part of cellular respiration. This entire process (Citric acid cycle) is necessary in producing one GTP or ATP, three NADHs, and two carbon dioxides. See also:<br /> | '''Succinyl-CoA synthetase''' or '''succinate-CoA ligase''' catalyzes the reversible reaction of succinyl-CoA + NDP + Pi <-> succinate + CoA + NTP (where N is either adenosine or guanosine. It can be found in Escherichia coli and is the fifth step in the [[Citric Acid Cycle|citric acid cycle]]. Due to its involvement in the citric acid cycle it is very important in all living cells that use oxygen as a part of cellular respiration. This entire process (Citric acid cycle) is necessary in producing one GTP or ATP, three NADHs, and two carbon dioxides. See also:<br /> | ||
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*[[Krebs cycle overview]] | *[[Krebs cycle overview]] | ||
*[[Krebs cycle reactions]] | *[[Krebs cycle reactions]] | ||
*[[Citric Acid Cycle]] | |||
*[[Krebs cycle step 5]] | *[[Krebs cycle step 5]] | ||
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==Regulation== | ==Regulation== | ||
Succinyl-CoA synthetase is not a major regulator in the Krebs cycle, making it dependent on the steps prior. However, there has been evidence that a high-affinity GDP-binding site does allosterically regulate the activity of the enzyme. It has also been seen that the binding of GDP to these allosteric sites actually leads to an increase in the rate of phosphorylation of Succinyl-Coa synthetase. This could be due to the fact that GDP is probably altering the affinity of the enzyme for GTP.<ref>Um, H.-D., and C. Klein. 1993. Evidence for allosteric regulation of succinyl-CoA synthetase. Biochem. J. 295:821–826.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134635/]</ref> | Succinyl-CoA synthetase is not a major regulator in the Krebs cycle, making it dependent on the steps prior. However, there has been evidence that a high-affinity GDP-binding site does allosterically regulate the activity of the enzyme. It has also been seen that the binding of GDP to these allosteric sites actually leads to an increase in the rate of phosphorylation of Succinyl-Coa synthetase. This could be due to the fact that GDP is probably altering the affinity of the enzyme for GTP.<ref>Um, H.-D., and C. Klein. 1993. Evidence for allosteric regulation of succinyl-CoA synthetase. Biochem. J. 295:821–826.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1134635/]</ref> | ||
==3D structures of succinyl-CoA synthetase== | ==3D structures of succinyl-CoA synthetase== | ||
[[Succinyl-CoA synthetase 3D structures]] | |||
</StructureSection> | |||
==Additional Resources== | ==Additional Resources== | ||
* [[Carbohydrate Metabolism]] <br /> | * [[Carbohydrate Metabolism]] <br /> | ||