Colicin Immunity Protein: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Gemma McGoldrick, Michal Harel, Alexander Berchansky, Jaime Prilusky

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-<StructureSection load='3gkl' size='350' scene='' caption='Colicin-E9 immunity protein (grey and green) complex with colicin-E7 (pink and yellow) and Zn+2 (PDB code [[3gkl]])' >
+<StructureSection load='3gkl' size='350' scene='' caption='Colicin-E9 immunity protein (grey) complex with colicin-E7 (pink) and Zn+2 ion (grey) (PDB code [[3gkl]])' >
+__TOC__
+==Function==
 '''Immunity proteins''' against [[Colicin]]s (EcCIP) are produced by ''E. coli'' alongside the relevant colicin protein (EcCol) to protect the cell from the cytotoxic domain of the colicin. Usually this involves binding to and blocking the active site of the domain, to prevent it from targeting the cells own mechanisms.
 Once released from the producing cell, the immunity proteins are no longer needed, as the cytotoxic domain needs to be active once a target cell has been penetrated. Often it is disassociated from the colicin upon binding to an outer membrane receptor on the target cell.  Find more details in<br />
-*[[Iia]]<br />
-*[[Imm]]<br />
 *[[ImmE1]]<br />
-*[[Im2]]<br />
+*[[Im2]]<ref>PMID:20479265</ref><br />
-*[[Im3]]<br />
+*[[Im3]]<ref>PMID:10574790</ref><br />
 *[[Im4]]<br />
-*[[ImmE5]]<br />
+*[[ImmE5]]<ref>PMID:17099236</ref><br />
 *[[ImmE6]]<br />
+*[[Colicin Immunity Protein 7]]<ref>PMID:10368275</ref><br />
 *[[Im7]]<br />
-*[[Colicin Immunity Protein 7]]<br />
 *[[Im8]]<br />
 *[[Im9]]<br />
-*[[Colicin Immunity Protein 9]]<br />
+*[[Colicin Immunity Protein 9]]<ref>PMID:18980319</ref><br />
 *[[Mutant immunity protein 9 (variant R12-13)]]<br />
 *[[Mutant immunity protein 9 (variant R12-2)]]<br />
-*[[ImmD]].
+*[[ImmD]].<ref>PMID:15014439</ref>
 ==Directed evolution and Colicin7/Immunity-proteins complexes<ref>PMID:19749752</ref>==
@@ Line 34: / Line 37: @@
 The <scene name='3gkl/Ali/1'>overall conformation</scene> of the two evolved variants <span style="color:lime;background-color:black;font-weight:bold;">R12-2</span> ([[3gkl]]) and <span style="color:cyan;background-color:black;font-weight:bold;">R12-13</span> ([[3gjn]]) is very similar. The variant <span style="color:lime;background-color:black;font-weight:bold;">R12-2</span> carries <scene name='3gkl/Ali/2'>mutation E41G</scene>. In the <span style="color:yellow;background-color:black;font-weight:bold;">bound wildtype Im9 (yellow)</span> Glu41 makes a <scene name='3gkl/Ali/3'>salt bridge</scene> with the <span style="color:orange;background-color:black;font-weight:bold;">ColE9’s</span> Lys97 ([[1bxi]]). While in the <font color='blueviolet'><b>R12-13</b></font>/<span style="color:cyan;background-color:black;font-weight:bold;">ColE7</span> complex the <font color='blueviolet'><b>closest ColE7 residues</b></font> <scene name='3gkl/Ali/4'>contacting</scene> <span style="color:cyan;background-color:black;font-weight:bold;">R12-13 Glu41</span> are <font color='blueviolet'><b>Thr531 (3.37Å) and Lys528 (8.85Å)</b></font> ([[3gjn]]). In the <span style="color:lime;background-color:black;font-weight:bold;">R12-2</span>/<font color='magenta'><b>ColE7</b></font> complex the <scene name='3gkl/Ali/5'>closest</scene> <font color='magenta'><b>ColE7 residue</b></font> to <span style="color:lime;background-color:black;font-weight:bold;">R12-2 Gly41</span> is <font color='magenta'><b>Thr531 (9.48Å)</b></font> ([[3gkl]]).
-</StructureSection>
 ==3D structure of Colicin immunity protein==
+[[Colicin immunity protein 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*Colicin-D immunity protein
-**[[1tfk]], [[1tfo]], [[1v74]] - EcCIPD + EcColD catalytic domain<br />
-*Colicin-E2 immunity protein
-**[[2no8]] – EcCIPE2 – NMR<BR />
-**[[2wpt]] – EcCIPE2 + EcColE9 (mutant)<br />
-**[[3u43]] - EcCIPE2 + EcColE2 DNase domain
-*Colicin-E3 immunity protein
-**[[3eip]] – EcCIPE3<BR />
-**[[1jch]] – EcCIPE3 + EcColE3<BR />
-**[[2b5u]] – EcCIPE3 + EcColE3 (mutant) <BR />
-**[[1e44]] – EcCIPE3 + EcColE3 nuclease domain<br />
-*Colicin-E5 immunity protein
-**[[2dfx]] – EcCIPE5 + EcColE5 C-terminal domain<br />
-**[[2fhz]] – EcCIPE5 + EcColE5 residues 74-180
-*Colicin-E7 immunity protein
-**[[1ayi]], [[1unk]], [[1cei]] – EcCIPE7<BR />
-**[[2k0d]] - EcCIPE7 - NMR<br />
-**[[1mz8]], [[7cei]] – EcCIPE7 + EcColE7 nuclease domain<br />
-**[[2jaz]], [[2jb0]], [[2jbg]], [[1znv]] – EcCIPE7 + EcColE7 nuclease domain (mutant) <BR />
-**[[2erh]], [[1ujz]] – EcCIPE7 (mutant) + EcColE7 (mutant)<br />
-**[[4f37]] – EcCIPE7 + IM7 immunity protein + antibody peptide<br />
-*Colicin-E8 immunity protein
-**[[1gxg]], [[1gxh]] – EcCIPE8 - NMR<BR />
-*Colicin-E9 immunity protein
-**[[1e0h]], [[1imp]], [[1imq]] – EcCIPE9 - NMR<BR />
-**[[2k5x]], [[1emv]], [[1bxi]] – EcCIPE9 + EcColE9 DNase domain<br />
-**[[2vln]], [[2vlo]], [[2vlp]], [[2vlq]] - EcCIPE9 + EcColE9 DNase domain (mutant) <BR />
-**[[2gze]], [[2gzg]], [[2gzi]], [[2gzj]], [[2gzf]], [[2gyk]], [[1fr2]] - EcCIPE9 (mutant) + EcColE9 DNase domain<br />
-**[[3gjn]] – EcCIPE9 (mutant) + EcColE7 nuclease domain<br />
-**[[3gkl]] – EcCIPE9 (mutant) + EcColE7 nuclease domain (mutant)
-*Colicin-M immunity protein
-**[[2xgl]], [[4aeq]] – EcCIPM  periplasmic domain<br />
+== References ==
-}}
+<references/>
 [[Category:Topic Page]]
 [[Category: Escherichia coli]]