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[[Image:3pto.png|left|200px|thumb|Crystal Structure of Nucleoprotein [[3pto]]]]
<StructureSection load='' size='350' side='right' caption='Nucleoprotein from Lassa virus trimer complex with TTP and Zn+2 ions (grey) [[3mx2]]' scene='46/461363/Cv/2' pspeed='8'>
{{STRUCTURE_3pto|  PDB=3pto  | SIZE=300| SCENE= |right|CAPTION=Nucleoprotein [[3pto]] }}


'''Nucleoproteins''' (NP) are associated with DNA or RNA.  NPs which contain RNA are called ribonucleoproteins (RNPs).  The NP RNA-recognition motif is named RRM.  The small nuclear ribonucleoproteins (snRNPs) are RNA-protein complexes which form part of the spliceosome.  The heterogenous nuclear ribonucleoproteins (hnRNP) are RNA-protein complexes which associates with the splicing apparatus and prevent the folding of pre-mRNA. The images at the left and at the right correspond to one representative NP, ''i.e.'' the crystal structure of Nucleoprotein from Vesicular stomatitis Indiana virus ([[3pto]]).
__TOC__
== Function ==


{{TOC limit|limit=2}}
'''Nucleoproteins''' (NP) are proteins associated with DNA or RNA<ref>PMID:2203758</ref>.  NPs which contain RNA are called '''ribonucleoproteins''' (RNPs).  The NP RNA-recognition motif is named RRM.<br />
<br />
The '''small nuclear ribonucleoproteins''' (snRNPs) are RNA-protein complexes which form part of the spliceosome<ref>PMID:15130578</ref>.  For details on SnRNP see [[Azoarcus group I intron]].<br />
<br />
The '''heterogenous nuclear ribonucleoproteins''' (hnRNP) are RNA-protein complexes which associates with the splicing apparatus and prevent the folding of pre-mRNA<ref>PMID:19099192</ref>. <br />
<br />
For details on '''heterogenous nuclear ribonucleoproteins''' see [[HnRNP A1]].<br />
<br />
For homology model and multiple sequence alignment see [[User:Michael Strong/H1N1/NP]] and [[User:Michael Strong/H1N1/NP/MSA]].<br />
<br />
For details on HIV-1 nucleoprotein see [[HIV-1 Nucleocapsid Protein (NC)]].
== 3D Structures of Nucleoprotein ==


===Nucleoprotein===
== Structural highlights ==  
*<scene name='46/461363/Cv/6'>TTP binding site</scene> in Nucleoprotein from Lassa virus ([[3mx2]]). Water molecules are shown as red spheres.
*<scene name='46/461363/Cv/7'>Zn coordination site</scene>.


[[3ouo]], [[3ov9]] – NP – Rift valley fever virus<br />
== 3D Structures of Nucleoprotein ==
[[3q7b]], [[3q7c]], [[3mwp]], [[3mwt]] – LvNP exonuclease domain – Lassa virus<br />
[[Nucleoprotein 3D structures]]
[[3pto]] - VvNP – Vesicular stomatitis Indiana virus<br />
[[2qvj]] – VvNP (mutant)<br />
[[2k48]] – NP N terminal – Andes virus - NMR<br />
[[3hd4]] – NP N terminal – Murine hepatitis virus<br />
[[2wfs]] – IvNP – Cryo EM – Influenza virus<br />
[[2q06]], [[2iqh]] – IvNP<br />
[[2i8b]] – NP C terminal – Ebola virus – NMR<br />
[[1yvr]] – XlNP Ro – ''Xenopus laevis''<br />
[[1wwf]] – MvNP P10 – Moloney murine leukemia virus – NMR<br />
[[2c86]] – BvNP N terminal – Avian infectious bronchitis virus<br />
[[2ca1]] – BvNP dimerization domain<br />
[[2btl]], [[2bxx]] – BvNP RRM (mutant)<br />
[[1n93]], [[1pp1]] – NP P40 – Borna disease virus<br />
 
===Nucleoprotein binary complex===
 
[[3mx2]], [[3mx5]] – LvNP + nucleotide<br />
[[3ptx]], [[3pu0]], [[3pu1]], [[3pu4]], [[2gic]] – VvNP + RNA<br />
[[2wyy]] – VvNP + poly U – Cryo EM<br />
[[3hhw]] – VvNP + phosphoprotein<br />
[[3hhz]] - VvNP + phosphoprotein + RNA<br />
[[2wj8]] – NP + RNA – Human respiratory syncytial virus<br />
[[2i91]], [[1yvp]] – XlNP Ro + RNA <br />
[[2gtt]] – NP + RNA – Rabies virus<br />
[[1wwg]], [[1wwd]], [[1wwe]], [[1u6p]] - MvNP P10 + RNA – NMR<br />
 
===Ribonucleoprotein===
 
[[2lbw]] – yRNP subunit 2 residues 36-156 (mutant) – yeast – NMR<br />
[[2lbx]] - yRNP subunit 2 residues 36-156 – NMR<br />
 
===Small nuclear ribonucleoprotein===
 
[[2a3j]] - h-snRNP U1 A RRM – human - NMR<br />
[[2l5i]], [[2l5j]] - h-snRNP U1 70kDa residues 131-151 – NMR<br />
[[2vy4]], [[2vy5]] - h-snRNP U11/U12 – NMR<br />
[[2cqj]] - h-snRNP U3 S4 domain – NMR<br />
[[1x4q]] - h-snRNP U4/U6 PWI domain - NMR<br />
[[2vrd]] - h-snRNP U1 C zinc finger domain - NMR<br />
[[2k3k]] – Dm-snRNP U1 A RRM1 – ''Drosophila melanogaster'' – NMR<br />
[[2aym]] - Dm-snRNP U1 A<br />
[[2b0g]] - Dm-snRNP U1 A RBD2 – NMR<br />
 
===Small nuclear ribonucleoprotein binary complex===
 
[[3plu]], [[3plv]] – y-snRNP HINDI domain + ubiquitin-like modifier HUB1<br />
[[2y9a]], [[2y9b]], [[2y9c]], [[2y9d]] – h-snRNP  U4 core domain<br />
[[3p49]] - h-snRNP A RRM + glycine riboswitch RNA<br />
[[3pgw]], [[3mum]], [[3mur]], [[3mut]], [[3muv]], [[3egz]] - h-snRNP U1 A + DNA riboswitch<br />
[[3mxh]], [[3irw]] - h-snRNP U1 A (mutant) + DNA riboswitch<br />
[[3k0j]] - h-snRNP U1 A (mutant) + RNA<br />
[[3iin]] - h-snRNP U1 A (mutant) + group I intron + DNA<br />
[[3b02]], [[3b03]], [[3b04]] - h-snRNP U1 A RRM1 + group I intron + RNA<br />
[[1zzn]] - h-snRNP U1 A RRM1 (mutant) + group I intron + RNA<br />
[[3l3c]], [[3g8s]], [[3g8t]], [[3g96]], [[3g9c]], [[2nz4]] - h-snRNP U1 A (mutant) + GLMS ribozyme + RNA<br />
[[3hhn]] - h-snRNP U1 A (mutant) + class I ligase ribozyme<br />
[[2oih]], [[2oj3]] - h-snRNP U1 A + HDV ribozyme<br />
[[3cul]], [[3cun]] - h-snRNP U1 A (mutant) + aminoacyl-tRNA synthetase ribozyme<br />
[[3cw1]] - h-snRNP U1 in spliceosome<br />
[[2ozb]] - h-snRNP U4/U6 + NHP2-like protein + RNA<br />
[[2bn5]] - Dm-snRNP U1 70kDa + PSI B box – NMR<br />
 
===Heterogenous nuclear ribonucleoprotein===
 
[[3s01]] – m-hnRNP L RRM3 domain – mouse<br />
[[2e5i]] - m-hnRNP L RRM – NMR<br />
[[2db1]] - m-hnRNP F RRM – NMR<br />
[[1x4b]] - h-hnRNP A2/B1 RRM – NMR<br />
[[1txp]] - h-hnRNP C oligomerization domain – NMR<br />
[[1wf2]] - h-hnRNP C1/C2 RRM – NMR<br />
[[2hgl]], [[2hgm]], [[2hgn]] - h-hnRNP F QRRM – NMR<br />
[[1wg5]], [[1wez]] - h-hnRNP H RRM – NMR<br />
[[2ad9]], [[2adb]], [[2adc]] - h-hnRNP I – NMR<br />
[[1zzi]], [[1zzj]] - h-hnRNP K KH domain + DNA<br />
[[1zzk]] - h-hnRNP K KH domain<br />
[[2do0]], [[2dh9]], [[2dgv]] - h-hnRNP M RRM – NMR<br />
[[2dgu]] - h-hnRNP Q RRM – NMR<br />
[[2dk2]] - h-hnRNP R RRM – NMR<br />


===Heterogenous nuclear ribonucleoprotein binary complex===
</StructureSection>


[[2kfy]], [[2kg0]], [[2kg1]] - h-hnRNP F QRRM + DNA – NMR<br />
== References ==
[[2z5n]], [[2z5o]], [[2ot8]] - h-hnRNP D0 C terminal + transportin-1<br />
<references/>
[[1u1k]], [[1u1l]], [[1u1m]], [[1u1n]], [[1u1o]], [[1u1p]], [[1u1q]], [[1u1r]] - h-hnRNP A1 + DNA<br />
[[Category:Topic Page]]
[[1wtb]], [[1x0f]] - h-hnRNP D0 RRM + DNA – NMR<br />

Latest revision as of 14:15, 9 January 2020


Function

Nucleoproteins (NP) are proteins associated with DNA or RNA[1]. NPs which contain RNA are called ribonucleoproteins (RNPs). The NP RNA-recognition motif is named RRM.

The small nuclear ribonucleoproteins (snRNPs) are RNA-protein complexes which form part of the spliceosome[2]. For details on SnRNP see Azoarcus group I intron.
The heterogenous nuclear ribonucleoproteins (hnRNP) are RNA-protein complexes which associates with the splicing apparatus and prevent the folding of pre-mRNA[3].
For details on heterogenous nuclear ribonucleoproteins see HnRNP A1.
For homology model and multiple sequence alignment see User:Michael Strong/H1N1/NP and User:Michael Strong/H1N1/NP/MSA.
For details on HIV-1 nucleoprotein see HIV-1 Nucleocapsid Protein (NC).

Structural highlights

  • in Nucleoprotein from Lassa virus (3mx2). Water molecules are shown as red spheres.
  • .

3D Structures of Nucleoprotein

Nucleoprotein 3D structures


Nucleoprotein from Lassa virus trimer complex with TTP and Zn+2 ions (grey) 3mx2

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Echols H. Nucleoprotein structures initiating DNA replication, transcription, and site-specific recombination. J Biol Chem. 1990 Sep 5;265(25):14697-700. PMID:2203758
  2. ↑ Yong J, Wan L, Dreyfuss G. Why do cells need an assembly machine for RNA-protein complexes? Trends Cell Biol. 2004 May;14(5):226-32. PMID:15130578 doi:http://dx.doi.org/10.1016/j.tcb.2004.03.010
  3. ↑ He Y, Smith R. Nuclear functions of heterogeneous nuclear ribonucleoproteins A/B. Cell Mol Life Sci. 2009 Apr;66(7):1239-56. doi: 10.1007/s00018-008-8532-1. PMID:19099192 doi:http://dx.doi.org/10.1007/s00018-008-8532-1

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