6mjg: Difference between revisions
New page: '''Unreleased structure''' The entry 6mjg is ON HOLD Authors: Ongpipattanakul, C., Nair, S.K. Description: Structure of dbOphMA in Complex with SAH and Methylated Peptide [[Category: U... |
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==Structure of dbOphMA in Complex with SAH and Methylated Peptide== | |||
<StructureSection load='6mjg' size='340' side='right'caption='[[6mjg]], [[Resolution|resolution]] 2.12Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6mjg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dendrothele_bispora_cbs_962.96 Dendrothele bispora cbs 962.96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MJG FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IML:N-METHYL-ISOLEUCINE'>IML</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=SAR:SARCOSINE'>SAR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mjg OCA], [http://pdbe.org/6mjg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mjg RCSB], [http://www.ebi.ac.uk/pdbsum/6mjg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mjg ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
N-methylation of nucleic acids, proteins, and peptides is a chemical modification with significant impact on biological regulation. Despite the simplicity of the structural change, N-methylation can influence diverse functions including epigenetics, protein complex formation, and microtubule stability. While there are limited examples of N-methylation of the alpha-amino group of bacterial and eukaryotic proteins, there are no examples of catalysts that carry out post-translation methylation of backbone amides in proteins or peptides. Recent studies have identified enzymes that catalyze backbone N-methylation on a peptide substrate, a reaction with little biochemical precedent, in a family of ribosomally synthesized natural products produced in basidiomycetes. Here, we describe the crystal structures of Dendrothele bispora dbOphMA, a methyltransferase that catalyzes multiple N-methylations on the peptide backbone. We further carry out biochemical studies of this catalyst to determine the molecular details that promote this unusual chemical transformation. The structural and biochemical framework described here could facilitate biotechnological applications of catalysts for the rapid production of backbone N-methylated peptides. | |||
Molecular Basis for Autocatalytic Backbone N-Methylation in RiPP Natural Product Biosynthesis.,Ongpipattanakul C, Nair SK ACS Chem Biol. 2018 Sep 25. doi: 10.1021/acschembio.8b00668. PMID:30204409<ref>PMID:30204409</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Nair, S | <div class="pdbe-citations 6mjg" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dendrothele bispora cbs 962 96]] | |||
[[Category: Large Structures]] | |||
[[Category: Nair, S K]] | |||
[[Category: Ongpipattanakul, C]] | [[Category: Ongpipattanakul, C]] | ||
[[Category: Biosynthetic protein]] | |||
[[Category: Borosin]] | |||
[[Category: Methyltransferase]] | |||
Latest revision as of 14:25, 1 January 2020
Structure of dbOphMA in Complex with SAH and Methylated PeptideStructure of dbOphMA in Complex with SAH and Methylated Peptide
Structural highlights
Publication Abstract from PubMedN-methylation of nucleic acids, proteins, and peptides is a chemical modification with significant impact on biological regulation. Despite the simplicity of the structural change, N-methylation can influence diverse functions including epigenetics, protein complex formation, and microtubule stability. While there are limited examples of N-methylation of the alpha-amino group of bacterial and eukaryotic proteins, there are no examples of catalysts that carry out post-translation methylation of backbone amides in proteins or peptides. Recent studies have identified enzymes that catalyze backbone N-methylation on a peptide substrate, a reaction with little biochemical precedent, in a family of ribosomally synthesized natural products produced in basidiomycetes. Here, we describe the crystal structures of Dendrothele bispora dbOphMA, a methyltransferase that catalyzes multiple N-methylations on the peptide backbone. We further carry out biochemical studies of this catalyst to determine the molecular details that promote this unusual chemical transformation. The structural and biochemical framework described here could facilitate biotechnological applications of catalysts for the rapid production of backbone N-methylated peptides. Molecular Basis for Autocatalytic Backbone N-Methylation in RiPP Natural Product Biosynthesis.,Ongpipattanakul C, Nair SK ACS Chem Biol. 2018 Sep 25. doi: 10.1021/acschembio.8b00668. PMID:30204409[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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