Pseudouridine synthase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky, Wayne Decatur, Angel Herraez

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-<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate   [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
+<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate   [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
-[[Pseudouridine]] is an abundant modified nucleotide found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the base of uridine. There are two major types of enzymes that catalyze this reaction:
+__TOC__
+==Function==
+[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:
-* [[RNA-guided Pseudoridine synthase]] -- Complexes of proteins and small RNAs have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to specify the site of modification through base-pairing with the target RNA molecule.
+* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.
-*  [[Guide-independent Pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through stand-alone enzymes to catalyze formation.
+*  [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.
 == Structural highlights ==
-The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
+The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
+== 3D Structures of pseudouridine synthase ==
+[[Pseudouridine synthase 3D structures]]
 </StructureSection>
+[[Category:Topic Page]]