|
|
| (8 intermediate revisions by 4 users not shown) |
| Line 1: |
Line 1: |
| <StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'> | | <StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'> |
| == Function == | | __TOC__ |
| '''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine<ref>PMID:14566049</ref>. '''Probable tRNA pseudouridine synthase''' (PPUS) is a similar enzyme. PUS are active in the ribosomal large subunit '''Ribosomal large subunit pseudouridine synthase''' and in the ribosomal small subunit '''Ribosomal small subunit pseudouridine synthase'''.<br />
| | ==Function== |
| Ribosomal large subunit pseudouridine synthases:<br />
| | [[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction: |
| *'''RluA''' is responsible for synthesis of pseudouridine from uracil in positions 746 in 23S ribosomal RNA and in the anticodon loop of tRNAPhe, tRNACys and tRNALeu<ref>PMID:17188032</ref>.<br />
| |
| *'''RluB''' is responsible for synthesis of pseudouridine from uracil in position 2605 in 23S ribosomal RNA<ref>PMID:24214967</ref>.<br />
| |
| *'''RluC''' is responsible for synthesis of pseudouridine from uracil in positions 955, 2504 and 2580 in 23S ribosomal RNA<ref>PMID:9660827</ref>.<br />
| |
| *'''RluD''' is responsible for synthesis of pseudouridine from uracil in positions 1911, 1915 and 1917 in 23S ribosomal RNA<ref>PMID:15928344</ref>.<br />
| |
| *'''RluE''' is responsible for synthesis of pseudouridine from uracil in position 2457 in 23S ribosomal RNA<ref>PMID:17320904</ref>.<br />
| |
| *'''RluF''' is responsible for synthesis of pseudouridine from uracil in position 2604 in 23S ribosomal RNA<ref>PMID:16712869</ref>.<br />
| |
|
| |
|
| Ribosomal small subunit pseudouridine synthase '''RsuA''' is responsible for synthesis of pseudouridine from uracil in positions 516 in 16S ribosomal RNA<ref>PMID:11953756</ref>.<br />
| | * [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule. |
| | |
| | * [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization. |
|
| |
|
| == Structural highlights == | | == Structural highlights == |
| The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]). | | The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]). |
| | == 3D Structures of pseudouridine synthase == |
| | [[Pseudouridine synthase 3D structures]] |
| | |
| </StructureSection> | | </StructureSection> |
| == 3D Structures of pseudouridine synthase==
| |
|
| |
| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| |
| {{#tree:id=OrganizedByTopic|openlevels=0|
| |
|
| |
| *tRNA pseudouridine synthase
| |
|
| |
| **[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
| |
| **[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br />
| |
| **[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br />
| |
| **[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
| |
| **[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
| |
| **[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
| |
|
| |
| *tRNA pseudouridine synthase complex with RNA
| |
|
| |
| **[[1k8w]] – EcTruB + stem-loop RNA<BR />
| |
| **[[1zl3]] - EcTruB (mutant) + stem-loop RNA<BR />
| |
| **[[2nqp]], [[2nr0]], [[2nre]] – EcTruA + Leu-tRNA<BR />
| |
| **[[1ze2]] - TmPUS B + RNA<BR />
| |
| **[[2ab4]] - TmPUS B (mutant) + RNA<BR />
| |
| **[[1r3e]], [[1r3f]] - EcTruB + RNA<BR />
| |
|
| |
| *Probable tRNA pseudouridine synthase
| |
|
| |
| **[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]], [[3hax]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<BR />
| |
| **[[3hay]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein<BR />
| |
| **[[2hvy]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA + small nuclear RNP protein + small nuclear RNP Gar1-like protein<BR />
| |
| **[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
| |
| **[[2rfk]] - PfPUS Cbf5 (mutant) + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
| |
| **[[2ey4]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein <BR />
| |
| **[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA<BR />
| |
| **[[2apo]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 - ''Methanocaldococcus jannaschii''<BR />
| |
|
| |
| *Ribosomal large subunit pseudouridine synthase
| |
|
| |
| **[[2i82]] - EcRluA catalytic domain + RNA<br />
| |
| **[[4lab]] - EcRluB catalytic domain <br />
| |
| **[[4lgt]] - EcRluB catalytic domain + RNA<br />
| |
| **[[1xpi]] - EcRluC catalytic domain<br />
| |
| **[[5vbb]] - hRluC-like<br />
| |
| **[[1prz]], [[1qyu]], [[1v9f]], [[1v9k]], [[2ist]] - EcRluD catalytic domain<br />
| |
| **[[2oml]], [[2olw]] - EcRluE catalytic domain<br />
| |
| **[[2gml]] - EcRluF catalytic domain<br />
| |
| **[[3dh3]] - EcRluF catalytic domain + RNA<br />
| |
|
| |
| *Ribosomal small subunit pseudouridine synthase
| |
|
| |
| **[[1vio]] - RsuA - ''Haemophilus influenzae''<br />
| |
| **[[1ksk]] - EcRsuA <br />
| |
| **[[1ksl]] - EcRsuA + uracil<br />
| |
| **[[1ksv]] - EcRsuA + UMP<br />
| |
|
| |
|
|
| |
|
| }}
| |
| == References ==
| |
| <references/>
| |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |