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| | <StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'> |
| | __TOC__ |
| | ==Function== |
| | [[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction: |
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| <StructureSection load='1ze2' size='340' side='right' caption='tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate [[1ze2]]' scene=''>
| | * [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule. |
| == Function ==
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| '''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine<ref>PMID:14566049</ref>. PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu). '''Probable tRNA pseudouridine synthase''' (PPUS) is a similar enzyme.
| | * [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization. |
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| == Structural highlights == | | == Structural highlights == |
| The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. | | The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]). |
| | == 3D Structures of pseudouridine synthase == |
| | [[Pseudouridine synthase 3D structures]] |
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| </StructureSection> | | </StructureSection> |
| == 3D Structures of tRNA pseudouridine synthase==
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *tRNA pseudouridine synthase
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| **[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
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| **[[1ksk]], [[1ksl]] – EcPUS RsuA<BR />
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| **[[1vio]] - PUS RsuA – ''Haemophilus influenzae''<BR />
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| **[[1xpi]] - EcPUS RluC catalytic domain<BR />
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| **[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <BR />
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| **[[2ist]] - EcPUS RluD <BR />
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| **[[1prz]], [[1qyu]], [[1v9f]] - EcPUS RluD catalytic domain<BR />
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| **[[2olw]] - EcPUS RluE<BR />
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| **[[2oml]] - EcPUS RluE catalytic domain<BR />
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| **[[2gml]] - EcPUS RluF catalytic domain<BR />
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| **[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br />
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| **[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br />
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| **[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
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| **[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
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| **[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
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| *tRNA pseudouridine synthase complex with RNA
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| **[[1k8w]] – EcPUS B + stem-loop RNA<BR />
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| **[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<BR />
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| **[[2i82]] – EcPUS RluA + RNA<BR />
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| **[[3dh3]] - EcPUS RluF + RNA<BR />
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| **[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<BR />
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| **[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<BR />
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| **[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
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| **[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA<BR />
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| **[[1ze2]] - TmPUS B + RNA<BR />
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| **[[2ab4]] - TmPUS B (mutant) + RNA<BR />
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| **[[1r3e]], [[1r3f]] - EcPUS B + RNA<BR />
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| *Probable tRNA pseudouridine synthase
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| **[[2apo]] – PPUS + ribosome biogenesis protein Nop10 – ''Methanocaldococcus jannaschii''<br />
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| **[[2ey4]] - PfPPUS Cbf5 + ribosome biogenesis protein Nop10 + small nucleolar RNP <br />
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| **[[2hvy]], [[3hax]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA<br />
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| **[[2rfk]] - PfPUS B + ribosome biogenesis protein Nop10 + small nucleolar RNP + RNA<br />
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| **[[3hay]] - PfPPUS B + ribosome biogenesis protein Nop10 + ribosomal protein L7 + small nucleolar RNP + RNA<br />
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| **[[2aus]] – PPUS B + ribosome biogenesis protein Nop10 – ''Pyrococcus abyssi''<br />
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| **[[1z2z]] – PPUS D – ''Methanosarcina mazei''<br />
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| }}
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| == References ==
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| <references/>
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| [[Category:Topic Page]] | | [[Category:Topic Page]] |