Pseudouridine synthase: Difference between revisions

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{{STRUCTURE_1k8w|  PDB=1k8w  | SIZE=400| SCENE= |right|CAPTION=E. coli tRNA pseudouridine synthase complex with stem-loop RNA, pseudouridine monophosphate and sulfate  [[1k8w]] }}
<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate   [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
__TOC__
==Function==
[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:


'''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine. PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu).
* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.


{{TOC limit|limit=2}}
*  [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.


== 3D Structures of tRNA pseudouridine synthase==
== Structural highlights ==
 
The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
== 3D Structures of pseudouridine synthase ==
[[1ze1]] – TmPUS B – ''Thermotoga maritima''<BR />
[[Pseudouridine synthase 3D structures]]
[[1ksk]], [[1ksl]] – EcPUS RsuA<BR /> 
[[1vio]] - PUS RsuA – ''Haemophilus influenzae''<BR />
[[1xpi]] - EcPUS RluC catalytic domain<BR />
[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <BR />
[[2ist]] - EcPUS RluD <BR />
[[1prz]], [[1qyu]], [[1v9f]] - EcPUS RluD catalytic domain<BR />
[[2olw]] - EcPUS RluE<BR />
[[2oml]] - EcPUS RluE catalytic domain<BR />
[[2gml]] - EcPUS RluF catalytic domain<BR />
[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
 
===PUS complex with RNA===  
 
[[1k8w]] – EcPUS B + stem-loop RNA<BR /> 
[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<BR />
[[2i82]] – EcPUS RluA + RNA<BR />
[[3dh3]] - EcPUS RluF + RNA<BR />
[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<BR />
[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS CBF5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA  – ''Pyrococcus furiosus''<BR />
[[3mqk]] - PfPUS CBF5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
[[3hjy]] - PfPUS CBF5 + ribosome biogenesis protein NOP10 + RNA<BR />
[[1ze2]] - TmPUS B + RNA<BR />
[[2ab4]] - TmPUS B (mutant) + RNA<BR />
[[1r3e]], [[1r3f]] - EcPUS B + RNA<BR />
 
[[2aus]] – PUS B + ribosome biogenesis protein Nop10 – ''Pyrococcus abyssi''


</StructureSection>




[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 12:20, 5 December 2019

Function

Pseudouridine is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:

  • RNA-guided pseudouridine synthase -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.

Structural highlights

The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase[1]. (1ze2).

3D Structures of pseudouridine synthase

Pseudouridine synthase 3D structures


tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate 1ze2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky, Wayne Decatur, Angel Herraez
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