Pseudouridine synthase: Difference between revisions

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Michal Harel, Alexander Berchansky, Jaime Prilusky, Wayne Decatur, Angel Herraez

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-{{STRUCTURE_1k8w|  PDB=1k8w  | SIZE=400| SCENE= |right|CAPTION=E. coli tRNA pseudouridine synthase complex with stem-loop RNA, pseudouridine monophosphate and sulfate  [[1k8w]] }}
+<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate   [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
+__TOC__
+==Function==
+[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:
-'''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine.  PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu).
+* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.
-{{TOC limit|limit=2}}
+*  [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.
-== 3D Structures of  tRNA pseudouridine synthase==
+== Structural highlights ==
+The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
-[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
+== 3D Structures of pseudouridine synthase ==
-[[1ze1]] – TmPUS B – ''Thermotoga maritima''<BR />
+[[Pseudouridine synthase 3D structures]]
-[[1ksk]], [[1ksl]] – EcPUS RsuA<BR />
-[[1vio]] - PUS RsuA – ''Haemophilus influenzae''<BR />
-[[1xpi]] - EcPUS RluC catalytic domain<BR />
-[[1v9k]] - EcPUS RluC C-terminal domain (mutant) <BR />
-[[2ist]] - EcPUS RluD <BR />
-[[1prz]], [[1qyu]], [[1v9f]] - EcPUS RluD catalytic domain<BR />
-[[2olw]] - EcPUS RluE<BR />
-[[2oml]] - EcPUS RluE catalytic domain<BR />
-[[2gml]] - EcPUS RluF catalytic domain<BR />
-[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
-[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
-[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
-===PUS complex with RNA===<BR />
-[[1k8w]] – EcPUS B + stem-loop RNA<BR />
-[[1zl3]] - EcPUS B (mutant) + stem-loop RNA<BR />
-[[2i82]] – EcPUS RluA + RNA<BR />
-[[3dh3]] - EcPUS RluF + RNA<BR />
-[[2nqp]], [[2nr0]], [[2nre]] – EcPUS TruA + Leu-tRNA<BR />
-[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS CBF5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA  – ''Pyrococcus furiosus''<BR />
-[[3mqk]] - PfPUS CBF5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
-[[3hjy]] - PfPUS CBF5 + ribosome biogenesis protein NOP10 + RNA<BR />
-[[1ze2]] - TmPUS B + RNA<BR />
-[[2ab4]] - TmPUS B (mutant) + RNA<BR />
-[[1r3e]], [[1r3f]] - EcPUS B + RNA<BR />
-[[2aus]] – PUS B + ribosome biogenesis protein Nop10 – ''Pyrococcus abyssi''
+</StructureSection>
 [[Category:Topic Page]]