Pseudouridine synthase: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky, Wayne Decatur, Angel Herraez

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+<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate   [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
+__TOC__
+==Function==
+[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:
-{{STRUCTURE_1k8w|  PDB=1k8w  | SIZE=400| SCENE= |right|CAPTION=E. coli tRNA pseudouridine synthase complex with stem-loop RNA  [[1k8w]] }}
+* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.
-'''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine.  PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu).
+*  [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.
-{{TOC limit|limit=2}}
+== Structural highlights ==
+The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
+== 3D Structures of pseudouridine synthase ==
+[[Pseudouridine synthase 3D structures]]
+</StructureSection>
-== 3D Structures of  tRNA pseudouridine synthase==
 [[Category:Topic Page]]