Maltose-binding protein: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
The <scene name='47/471830/Cv/4'>MBP sugar binding cleft is rich with protein-ligand hydrogen bonds</scene><ref>PMID:9309217</ref>. Water molecules shown as red spheres.
The <scene name='47/471830/Cv/5'>MBP sugar binding cleft is rich with protein-ligand hydrogen bonds</scene><ref>PMID:9309217</ref>. Water molecules are shown as red spheres.
</StructureSection>
 
==3D structures of maltose-binding protein==
==3D structures of maltose-binding protein==
[[Maltose-binding protein 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*'''MBP'''
 
**[[1llx]], [[1jw4]], [[1lls]], [[1omp]] - MBP<br />
**[[3sey]], [[3sex]], [[3sew]], [[3sev]], [[3seu]], [[3set]], [[3ses]], [[3ser]], [[2v93]], [[1zkb]], [[1n3x]], [[1peb ]], [[1mpc]], [[3kjt]], [[1mdp]], [[1mdq]] -  MBP (mutant)<br />
**[[2klf]], [[2d21]] – MBP – NMR<br />
**[[1ziu]] – MBP (mutant) + Ni<br />
**[[1zjl]] – MBP (mutant) + Zn<br />
**[[1zmg]] - MBP (mutant) + Cu<br />
**[[4log]] - MBP /nuclear receptor<br />
**[[4ogm]] - MBP /pilin<br />
**[[4pe2]] - MBP /pilA1<br />
**[[4khz]] - MBP  MalK, MalF, MalG, MBP <br />
**[[3fh6]] - MBP  MalK, MalF, MalG <br />
**[[2xd2]] – SpMBP – ''Streptococcus pneumoniae''
 
*MBP complex with saccharide
 
**[[1n3w]], [[1nl5]], [[1mpd]], [[1iud]], [[1jvx]], [[1jvy]] - MBP (mutant) + maltose<br />
**[[1anf]], [[1jw5]], [[2xz3]] - MBP + maltose<br />
**[[3mbp]] - MBP + maltotriose<br />
**[[4mbp]] - MBP + maltotetrose<br />
**[[1ez9]] - MBP + maltodextrin<br />
**[[3hpi]] – MBP (mutant) + sucrose<br />
**[[1lax]] - MBP (mutant) + glucose<br />
**[[2h25]], [[1ezo]], [[1dmb]] - MBP + β-cyclodextrin<br />
**[[1ezp]] - MBP (mutant) + β-cyclodextrin<br />
**[[1fqa]], [[1fqb]], [[1fqc]], [[1fqd]] - MBP + maltotetraitol<br />
**[[3puy]], [[3puz]] - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP<br />
**[[3rlf]] - MBP  MalK, MalF, MalG, MBP + maltose + AMPPNP<br />
**[[4jbw]] - MBP  MalK, MalF, MalG + EIIAGLC<br />
**[[3pv0]] - MBP  MalK, MalF, MalG, MBP + maltose <br />
**[[2r6g]], [[3puv]], [[3puw]], [[3pux]], [[4ki0]] - MBP  MalK, MalF, MalG, MBP + maltose + nucleotide<br />
**[[3ob4]] - MBP  AraH2 (mutant) + maltotriose<br />
**[[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br />
**[[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br />
**[[2xd3]] – SpMBP + maltopentaose
 
*MBP complex with protein


**[[3pgf]] – MBP (mutant) + synthetic antigen binder<br />
**[[2r6g]], [[4khz]], [[4ki0]] - MBP + maltose transporter complex<br />
**[[3puv]], [[3puw]], [[3pux]] – MBP + maltose transporter complex + ADP <br />
**[[3rlf]] - MBP + maltose transporter complex + Mg-AMPPNP<br />
**[[1svx]] – MBP + ankyrin repeat protein OFF7<br />
**[[1nmu]] - MBP + ribosomal protein L30<br />
**[[1t0k]] - MBP + ribosomal protein L30 + RNA<br />
**[[3rum]] – MBP + ristocetin<br />
**[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br />
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 11:22, 28 October 2019

Function

Maltose-binding protein (MBP) or ABC maltose transporter is part of the maltose/maltodextrin system in E. coli which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport[1].

Relevance

MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in E. coli by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose[2].

Structural highlights

The [3]. Water molecules are shown as red spheres.

3D structures of maltose-binding protein

Maltose-binding protein 3D structures


E. coli maltose-binding protein complex with maltose (PDB entry 1anf)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 1999 Aug;8(8):1668-74. PMID:10452611 doi:http://dx.doi.org/10.1110/ps.8.8.1668
  2. ↑ Sun P, Tropea JE, Waugh DS. Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner. Methods Mol Biol. 2011;705:259-74. doi: 10.1007/978-1-61737-967-3_16. PMID:21125392 doi:http://dx.doi.org/10.1007/978-1-61737-967-3_16
  3. ↑ Quiocho FA, Spurlino JC, Rodseth LE. Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor. Structure. 1997 Aug 15;5(8):997-1015. PMID:9309217

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Alexander Berchansky, Michal Harel
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